Induction of alternative oxidase by excess copper in sycamore cell suspensions

Mário Pádua; Serge Aubert; Adalcina Casimiro; Richard Bligny; A. Harvey Millar; David A. Day

doi:10.1016/S0981-9428(99)80074-6

Induction of alternative oxidase by excess copper in sycamore cell suspensions

Mário Pádua, Serge Aubert, Adalcina Casimiro, Richard Bligny, A. Harvey Millar, David A. Day

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Sycamore suspension cells (Acer pseudoplatanus L.) were used to investigate the effect of copper on respiratory electron transport. Alternative oxidase (AOX) protein content and enzymatic capacity increased as a function of the concentration of copper added to the culture medium, from 0.2 to 50 μM. The latter sublethal concentration, which arrests mitochondrial biogenesis and thereby decreases cell respiration rates, stimulated cyanide-insensitive oxygen uptake in cells and mitochondria isolated therefrom. This was correlated with the accumulation of two proteins (30 and 36 kDa) which reacted with monoclonal antibodies against AOX. An accumulation of a 1.6-kb AOX mRNA was also observed. The possible mechanisms through which copper affects AOX are discussed.

Original language	English
Pages (from-to)	131-137
Number of pages	7
Journal	Plant Physiology and Biochemistry
Volume	37
Issue number	2
DOIs	https://doi.org/10.1016/S0981-9428(99)80074-6
Publication status	Published - 1 Jan 1999
Externally published	Yes

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title = "Induction of alternative oxidase by excess copper in sycamore cell suspensions",

abstract = "Sycamore suspension cells (Acer pseudoplatanus L.) were used to investigate the effect of copper on respiratory electron transport. Alternative oxidase (AOX) protein content and enzymatic capacity increased as a function of the concentration of copper added to the culture medium, from 0.2 to 50 μM. The latter sublethal concentration, which arrests mitochondrial biogenesis and thereby decreases cell respiration rates, stimulated cyanide-insensitive oxygen uptake in cells and mitochondria isolated therefrom. This was correlated with the accumulation of two proteins (30 and 36 kDa) which reacted with monoclonal antibodies against AOX. An accumulation of a 1.6-kb AOX mRNA was also observed. The possible mechanisms through which copper affects AOX are discussed.",

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T1 - Induction of alternative oxidase by excess copper in sycamore cell suspensions

AU - Pádua, Mário

AU - Aubert, Serge

AU - Casimiro, Adalcina

AU - Bligny, Richard

AU - Millar, A. Harvey

AU - Day, David A.

PY - 1999/1/1

Y1 - 1999/1/1

N2 - Sycamore suspension cells (Acer pseudoplatanus L.) were used to investigate the effect of copper on respiratory electron transport. Alternative oxidase (AOX) protein content and enzymatic capacity increased as a function of the concentration of copper added to the culture medium, from 0.2 to 50 μM. The latter sublethal concentration, which arrests mitochondrial biogenesis and thereby decreases cell respiration rates, stimulated cyanide-insensitive oxygen uptake in cells and mitochondria isolated therefrom. This was correlated with the accumulation of two proteins (30 and 36 kDa) which reacted with monoclonal antibodies against AOX. An accumulation of a 1.6-kb AOX mRNA was also observed. The possible mechanisms through which copper affects AOX are discussed.

AB - Sycamore suspension cells (Acer pseudoplatanus L.) were used to investigate the effect of copper on respiratory electron transport. Alternative oxidase (AOX) protein content and enzymatic capacity increased as a function of the concentration of copper added to the culture medium, from 0.2 to 50 μM. The latter sublethal concentration, which arrests mitochondrial biogenesis and thereby decreases cell respiration rates, stimulated cyanide-insensitive oxygen uptake in cells and mitochondria isolated therefrom. This was correlated with the accumulation of two proteins (30 and 36 kDa) which reacted with monoclonal antibodies against AOX. An accumulation of a 1.6-kb AOX mRNA was also observed. The possible mechanisms through which copper affects AOX are discussed.

KW - Acer pseudoplatanus

KW - Alternative oxidase

KW - Copper

KW - Plant metabolism

KW - Respiration

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U2 - 10.1016/S0981-9428(99)80074-6

DO - 10.1016/S0981-9428(99)80074-6

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SN - 0981-9428

VL - 37

SP - 131

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JO - Plant Physiology and Biochemistry

JF - Plant Physiology and Biochemistry

IS - 2

ER -

Induction of alternative oxidase by excess copper in sycamore cell suspensions

Abstract

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