Increased Tolerance to Mn Deficiency in Transgenic Tobacco Overproducing Superoxide Dismutase

Q. Yu, L.D. Osborne, Zed Rengel

    Research output: Contribution to journalArticle

    24 Citations (Scopus)

    Abstract

    The effect of Mn deficiency on plant growth and activities of superoxide dismutase (SOD) was studied in hydroponically-grown seedlings of transgenic tobacco (Nicotiana tabacum L.) engineered to overexpress FeSOD in chloroplasts or MnSOD in chloroplasts or mitochondria. In comparison to the non-transgenic parental line, the activity of MnSOD in the lines overproducing MnSOD was 1.6-fold greater, and the activity of FeSOD in the FeSOD-overproducing lines was 3.2-fold greater, regardless of the Mn treatment (deficient or sufficient). The MnSOD activities decreased due to Mn deficiency, while activities of FeSOD and Cu/ZnSOD remained unaffected 25 d after transplanting (DAT). With an increased duration of the Mn deficiency stress (45 DAT), FeSOD activity decreased, and that of MnSOD continued to decrease, while Cu/ZnSOD activity simultaneously increased. Under Mn sufficiency, non-transgenic parental plants had greater shoot biomass than the transgenics; however, when subjected to Mn deficiency stress, non-transgenic parents suffered a proportionally greater growth reduction than transgenic lines. Thus, overproduction of MnSOD in chloroplasts may provide protection from oxidative stress caused by Mn deficiency. (C) 1999 Annals of Botany Company.
    Original languageEnglish
    Pages (from-to)543-547
    JournalAnnals of Botany
    Volume84
    DOIs
    Publication statusPublished - 1999

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