Projects per year
Abstract
Recent studies in Arabidopsis (Arabidopsis thaliana) have reported conflicting roles for NAC DOMAIN CONTAINING PROTEIN 17 (ANAC017), a transcription factor regulating mitochondria-to-nuclear signalling, and its closest paralog NAC DOMAIN CONTAINING PROTEIN 16 (ANAC016), in leaf senescence. By synchronising senescence in individually darkened leaves of knock-out and overexpressing mutants from these contrasting studies, we demonstrate that elevated ANAC017 expression consistently causes accelerated senescence and cell death. A time-resolved transcriptome analysis revealed that senescence-associated pathways such as autophagy are not constitutively activated in ANAC017 overexpression lines, but require a senescence-stimulus to trigger accelerated induction. ANAC017 transcript and ANAC017-target genes are constitutively upregulated in ANAC017 overexpression lines, but surprisingly show a transient 'super-induction' one day after senescence-induction. This induction of ANAC017 and its target genes is observed during the later stages of age-related and dark induced senescence, indicating the ANAC017 pathway is also activated in natural senescence. In contrast, knockout mutants of ANAC017 showed lowered senescence-induced induction of ANAC017 target genes during the late stages of dark-induced senescence. Finally, promotor binding analyses show that the ANAC016 promoter sequence is directly bound by ANAC017, so ANAC016 likely acts downstream of ANAC017 and is directly transcriptionally controlled by ANAC017 in a feed-forward loop during late senescence.
Original language | English |
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Pages (from-to) | 2205–2221 |
Number of pages | 17 |
Journal | Plant Physiology |
Volume | 186 |
Issue number | 4 |
DOIs | |
Publication status | Published - Aug 2021 |
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Dive into the research topics of 'Increased expression of ANAC017 primes for accelerated senescence'. Together they form a unique fingerprint.Projects
- 2 Finished
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In touch with the environment: dissecting early tactile responses in plants
Millar, H. (Investigator 01), Singh, K. (Investigator 02) & Van Aken, O. (Investigator 03)
ARC Australian Research Council
1/01/16 → 31/03/19
Project: Research
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ARC Centre of Excellence in Plant Energy Biology 2014 (CPEB2)
Millar, H. (Investigator 01), Pogson, B. (Investigator 02), Tyerman, S. (Investigator 03), Small, I. (Investigator 04), Whelan, J. (Investigator 05), Borevitz, J. (Investigator 06), Lister, R. (Investigator 07), Atkin, O. (Investigator 08) & Munns, R. (Investigator 09)
ARC Australian Research Council
1/01/14 → 31/05/21
Project: Research