In vivo label-free lymphangiography of cutaneous lymphatic vessels in human burn scars using optical coherence tomography

Peijun Gong, Shaghayegh Es’haghian, Karl Anton Harms, Alexandra Murray, Suzanne Rea, Fiona M. Wood, David D. Sampson, Robert A. McLaughlin

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

We present an automated, label-free method for lymphangiography of cutaneous lymphatic vessels in humans in vivo using optical coherence tomography (OCT). This method corrects for the variation in OCT signal due to the confocal function and sensitivity fall-off of a spectral-domain OCT system and utilizes a single-scattering model to compensate for Ascan signal attenuation to enable reliable thresholding of lymphatic vessels. A segmentjoining algorithm is then incorporated into the method to mitigate partial-volume effects with small vessels. The lymphatic vessel images are augmented with images of the blood vessel network, acquired from the speckle decorrelation with additional weighting to differentiate blood vessels from the observed high decorrelation in lymphatic vessels. We demonstrate the method with longitudinal scans of human burn scar patients undergoing ablative fractional laser treatment, showing the visualization of the cutaneous lymphatic and blood vessel networks.

Original languageEnglish
Article number273156
Pages (from-to)4886-4898
Number of pages13
JournalBiomedical Optics Express
Volume7
Issue number12
DOIs
Publication statusPublished - 1 Dec 2016

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