In vitro assessment of the biological activity of basic fibroblast growth factor released from various polymers and biomatrices

Marilyn Davies; C.A. Mitchell; Moira Maley; Miranda Grounds; Alan Harvey; G.W. Plant; D.J. Wood; Y. Hong; T.V. Chirila

In vitro assessment of the biological activity of basic fibroblast growth factor released from various polymers and biomatrices

Marilyn Davies, C.A. Mitchell, Moira Maley, Miranda Grounds, Alan Harvey, G.W. Plant, D.J. Wood, Y. Hong, T.V. Chirila

Research output: Contribution to journal › Article › peer-review

Abstract

The kinetics of controlled release of basic fibroblast growth factor (bFGF) from polymers (sutures, polycarbonate, Hydron, and Elvax), biopolymers (alginate), and biomatrices (lens capsules), and conditions for storage of bFGF (temperature, plastic type, heparin) were evaluated in vitro. Tissue culture proliferation bioassays with 3T3 fibroblasts, showed that only lens capsules with bFGF had a sustained release of bFGF for up to three weeks. The other materials released all of the 'bound' bFGF with two hours or produced an inflammatory response in vivo. Therefore, the lens tissue had the most potential for controlled long-term delivery of bFGF in vivo. These studies emphasise the importance of in vitro analysis of release kinetics of growth factors from a range of materials as a basis for potential in vivo applications.

Original language	English
Pages (from-to)	31-56
Journal	Journal of Biomaterials Applications
Volume	12
Publication status	Published - 1997

Cite this

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title = "In vitro assessment of the biological activity of basic fibroblast growth factor released from various polymers and biomatrices",

abstract = "The kinetics of controlled release of basic fibroblast growth factor (bFGF) from polymers (sutures, polycarbonate, Hydron, and Elvax), biopolymers (alginate), and biomatrices (lens capsules), and conditions for storage of bFGF (temperature, plastic type, heparin) were evaluated in vitro. Tissue culture proliferation bioassays with 3T3 fibroblasts, showed that only lens capsules with bFGF had a sustained release of bFGF for up to three weeks. The other materials released all of the 'bound' bFGF with two hours or produced an inflammatory response in vivo. Therefore, the lens tissue had the most potential for controlled long-term delivery of bFGF in vivo. These studies emphasise the importance of in vitro analysis of release kinetics of growth factors from a range of materials as a basis for potential in vivo applications.",

author = "Marilyn Davies and C.A. Mitchell and Moira Maley and Miranda Grounds and Alan Harvey and G.W. Plant and D.J. Wood and Y. Hong and T.V. Chirila",

year = "1997",

language = "English",

volume = "12",

pages = "31--56",

journal = "Journal of Biomaterials Applications",

issn = "0885-3282",

publisher = "SAGE Publications Ltd",

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TY - JOUR

T1 - In vitro assessment of the biological activity of basic fibroblast growth factor released from various polymers and biomatrices

AU - Davies, Marilyn

AU - Mitchell, C.A.

AU - Maley, Moira

AU - Grounds, Miranda

AU - Harvey, Alan

AU - Plant, G.W.

AU - Wood, D.J.

AU - Hong, Y.

AU - Chirila, T.V.

PY - 1997

Y1 - 1997

N2 - The kinetics of controlled release of basic fibroblast growth factor (bFGF) from polymers (sutures, polycarbonate, Hydron, and Elvax), biopolymers (alginate), and biomatrices (lens capsules), and conditions for storage of bFGF (temperature, plastic type, heparin) were evaluated in vitro. Tissue culture proliferation bioassays with 3T3 fibroblasts, showed that only lens capsules with bFGF had a sustained release of bFGF for up to three weeks. The other materials released all of the 'bound' bFGF with two hours or produced an inflammatory response in vivo. Therefore, the lens tissue had the most potential for controlled long-term delivery of bFGF in vivo. These studies emphasise the importance of in vitro analysis of release kinetics of growth factors from a range of materials as a basis for potential in vivo applications.

AB - The kinetics of controlled release of basic fibroblast growth factor (bFGF) from polymers (sutures, polycarbonate, Hydron, and Elvax), biopolymers (alginate), and biomatrices (lens capsules), and conditions for storage of bFGF (temperature, plastic type, heparin) were evaluated in vitro. Tissue culture proliferation bioassays with 3T3 fibroblasts, showed that only lens capsules with bFGF had a sustained release of bFGF for up to three weeks. The other materials released all of the 'bound' bFGF with two hours or produced an inflammatory response in vivo. Therefore, the lens tissue had the most potential for controlled long-term delivery of bFGF in vivo. These studies emphasise the importance of in vitro analysis of release kinetics of growth factors from a range of materials as a basis for potential in vivo applications.

M3 - Article

SN - 0885-3282

VL - 12

SP - 31

EP - 56

JO - Journal of Biomaterials Applications

JF - Journal of Biomaterials Applications

ER -

In vitro assessment of the biological activity of basic fibroblast growth factor released from various polymers and biomatrices

Abstract

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