In Vitro 3D Mechanical Stimulation to Tendon-Derived Stem Cells by Bioreactor

Ziming Chen; Peilin Chen; Rui Ruan; Minghao Zheng

doi:10.1007/7651_2021_432

In Vitro 3D Mechanical Stimulation to Tendon-Derived Stem Cells by Bioreactor

Ziming Chen, Peilin Chen, Rui Ruan, Minghao Zheng

Research output: Chapter in Book/Conference paper › Chapter › peer-review

4 Citations (Scopus)

Abstract

Bioreactors can offer an advanced platform to provide conditions that mimic the native microenvironment, which can also provide stretching environment for mechanobiology research. Tendon-derived stem cells (TDSCs) are a type of mechanosensitive and multipotent cells, which behave differently in diverse mechanical stretching environments. We have proved the in vitro three-dimensional (3D) mechanical stimulation could closely mimic the stretching environment in vivo. Thus, here we describe applying a customized bioreactor to provide 3D force for mechanical stimulation on TDSC in vitro.

Original language	English
Title of host publication	Bioreactors in Stem Cell Biology
Subtitle of host publication	Methods and Protocols
Editors	Kursad Turksen
Place of Publication	USA
Publisher	Humana Press Inc.
Pages	135-144
Number of pages	10
Edition	2nd
ISBN (Electronic)	978-1-0716-2018-2
ISBN (Print)	978-1-0716-2017-5
DOIs	https://doi.org/10.1007/7651_2021_432
Publication status	Published - 2022

Publication series

Name	Methods in Molecular Biology
Volume	2436
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

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10.1007/7651_2021_432

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title = "In Vitro 3D Mechanical Stimulation to Tendon-Derived Stem Cells by Bioreactor",

abstract = "Bioreactors can offer an advanced platform to provide conditions that mimic the native microenvironment, which can also provide stretching environment for mechanobiology research. Tendon-derived stem cells (TDSCs) are a type of mechanosensitive and multipotent cells, which behave differently in diverse mechanical stretching environments. We have proved the in vitro three-dimensional (3D) mechanical stimulation could closely mimic the stretching environment in vivo. Thus, here we describe applying a customized bioreactor to provide 3D force for mechanical stimulation on TDSC in vitro.",

keywords = "Bioreactor, Mechanical loading, Mechanobiology, RT-qPCR, Tendon formation",

author = "Ziming Chen and Peilin Chen and Rui Ruan and Minghao Zheng",

note = "Funding Information: The authors acknowledge financial support from Australian Research Council Industrial Transformation Training Centre for Personalised Therapeutics Technologies (IC170100016). Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC.",

year = "2022",

doi = "10.1007/7651_2021_432",

language = "English",

isbn = "978-1-0716-2017-5",

series = "Methods in Molecular Biology",

publisher = "Humana Press Inc.",

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booktitle = "Bioreactors in Stem Cell Biology",

address = "United States",

edition = "2nd",

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In Vitro 3D Mechanical Stimulation to Tendon-Derived Stem Cells by Bioreactor. / Chen, Ziming; Chen, Peilin ; Ruan, Rui et al.
Bioreactors in Stem Cell Biology: Methods and Protocols. ed. / Kursad Turksen. 2nd. ed. USA: Humana Press Inc., 2022. p. 135-144 (Methods in Molecular Biology; Vol. 2436).

Research output: Chapter in Book/Conference paper › Chapter › peer-review

TY - CHAP

T1 - In Vitro 3D Mechanical Stimulation to Tendon-Derived Stem Cells by Bioreactor

AU - Chen, Ziming

AU - Chen, Peilin

AU - Ruan, Rui

AU - Zheng, Minghao

N1 - Funding Information: The authors acknowledge financial support from Australian Research Council Industrial Transformation Training Centre for Personalised Therapeutics Technologies (IC170100016). Publisher Copyright: © 2021, Springer Science+Business Media, LLC.

PY - 2022

Y1 - 2022

N2 - Bioreactors can offer an advanced platform to provide conditions that mimic the native microenvironment, which can also provide stretching environment for mechanobiology research. Tendon-derived stem cells (TDSCs) are a type of mechanosensitive and multipotent cells, which behave differently in diverse mechanical stretching environments. We have proved the in vitro three-dimensional (3D) mechanical stimulation could closely mimic the stretching environment in vivo. Thus, here we describe applying a customized bioreactor to provide 3D force for mechanical stimulation on TDSC in vitro.

AB - Bioreactors can offer an advanced platform to provide conditions that mimic the native microenvironment, which can also provide stretching environment for mechanobiology research. Tendon-derived stem cells (TDSCs) are a type of mechanosensitive and multipotent cells, which behave differently in diverse mechanical stretching environments. We have proved the in vitro three-dimensional (3D) mechanical stimulation could closely mimic the stretching environment in vivo. Thus, here we describe applying a customized bioreactor to provide 3D force for mechanical stimulation on TDSC in vitro.

KW - Bioreactor

KW - Mechanical loading

KW - Mechanobiology

KW - RT-qPCR

KW - Tendon formation

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U2 - 10.1007/7651_2021_432

DO - 10.1007/7651_2021_432

M3 - Chapter

C2 - 34505270

AN - SCOPUS:85123411502

SN - 978-1-0716-2017-5

T3 - Methods in Molecular Biology

SP - 135

EP - 144

BT - Bioreactors in Stem Cell Biology

A2 - Turksen, Kursad

PB - Humana Press Inc.

CY - USA

ER -

In Vitro 3D Mechanical Stimulation to Tendon-Derived Stem Cells by Bioreactor

Abstract

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Mechanotransduction plays an essential role in maintaining tendon homeostasis

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