In situ seed development and in vitro regeneration of three difficult-to-propagate Lepidosperma species (Cyperaceae)

A. Kodym, Shane Turner, J. Delpratt

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    Field studies of fruit production from Lepidosperma concavum R.Br., L. laterale R.Br. and L. longitudinale Labill. showed that large proportions (21-77%) of fruits were unfilled and that filled and unfilled fruits looked alike. Bagging of inflorescences demonstrated that filled fruits tended to be shed, while empty fruits remained within the inflorescence. Time of collection was critical for obtaining viable seeds, with successful harvesting limited to a short period (weeks) after maturation. The timing of flowering and fruit maturation were fairly consistent between species, populations and years in our study area. In L. concavum fruit production was increased in cultivation compared with wild populations. In all three species, very little or no germination of fruits occurred under nursery conditions. In vitro culture initiation was attempted using intact fruits, nicked fruits and seeds on 1/2MS (Murashige and Skoog) medium with 1 μM zeatin and 0.5M gibberellic acid in darkness. Culture of intact fruit resulted in no germination, while nicked fruit showed some germination response. Best results were achieved from seeds with germination occurring as early as 7 to 18 days depending on the species. Germination of L. concavum, L. laterale and L. longitudinale was 86%, 64% and 83% respectively within 5 weeks. Germination response was strongly influenced by seed maturity. Mature seeds germinated significantly faster than immature seeds. On a small proportion of cultured seeds, calli formed and differentiated into numerous plantlets on growth regulator-free medium. Given the promising results observed in this study, in vitro culture appears to be a practical means of mass propagating Lepidosperma species. © 2010 CSIRO.
    Original languageEnglish
    Pages (from-to)107-114
    JournalAustralian Journal of Botany
    Volume58
    Issue number2
    DOIs
    Publication statusPublished - 2010

    Fingerprint

    in vitro regeneration
    Cyperaceae
    seed development
    fruit
    regeneration
    seed
    fruits
    germination
    seeds
    fruit production
    in vitro culture
    fruiting
    inflorescences
    maturation
    in situ
    zeatin
    growth regulator
    fruit growing
    growth regulators
    gibberellic acid

    Cite this

    @article{73534cb0256845aca3d9cb5514a9e51c,
    title = "In situ seed development and in vitro regeneration of three difficult-to-propagate Lepidosperma species (Cyperaceae)",
    abstract = "Field studies of fruit production from Lepidosperma concavum R.Br., L. laterale R.Br. and L. longitudinale Labill. showed that large proportions (21-77{\%}) of fruits were unfilled and that filled and unfilled fruits looked alike. Bagging of inflorescences demonstrated that filled fruits tended to be shed, while empty fruits remained within the inflorescence. Time of collection was critical for obtaining viable seeds, with successful harvesting limited to a short period (weeks) after maturation. The timing of flowering and fruit maturation were fairly consistent between species, populations and years in our study area. In L. concavum fruit production was increased in cultivation compared with wild populations. In all three species, very little or no germination of fruits occurred under nursery conditions. In vitro culture initiation was attempted using intact fruits, nicked fruits and seeds on 1/2MS (Murashige and Skoog) medium with 1 μM zeatin and 0.5M gibberellic acid in darkness. Culture of intact fruit resulted in no germination, while nicked fruit showed some germination response. Best results were achieved from seeds with germination occurring as early as 7 to 18 days depending on the species. Germination of L. concavum, L. laterale and L. longitudinale was 86{\%}, 64{\%} and 83{\%} respectively within 5 weeks. Germination response was strongly influenced by seed maturity. Mature seeds germinated significantly faster than immature seeds. On a small proportion of cultured seeds, calli formed and differentiated into numerous plantlets on growth regulator-free medium. Given the promising results observed in this study, in vitro culture appears to be a practical means of mass propagating Lepidosperma species. {\circledC} 2010 CSIRO.",
    author = "A. Kodym and Shane Turner and J. Delpratt",
    year = "2010",
    doi = "10.1071/BT09183",
    language = "English",
    volume = "58",
    pages = "107--114",
    journal = "Australian Journal of Botany",
    issn = "0067-1924",
    publisher = "CSIRO Publishing",
    number = "2",

    }

    In situ seed development and in vitro regeneration of three difficult-to-propagate Lepidosperma species (Cyperaceae). / Kodym, A.; Turner, Shane; Delpratt, J.

    In: Australian Journal of Botany, Vol. 58, No. 2, 2010, p. 107-114.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - In situ seed development and in vitro regeneration of three difficult-to-propagate Lepidosperma species (Cyperaceae)

    AU - Kodym, A.

    AU - Turner, Shane

    AU - Delpratt, J.

    PY - 2010

    Y1 - 2010

    N2 - Field studies of fruit production from Lepidosperma concavum R.Br., L. laterale R.Br. and L. longitudinale Labill. showed that large proportions (21-77%) of fruits were unfilled and that filled and unfilled fruits looked alike. Bagging of inflorescences demonstrated that filled fruits tended to be shed, while empty fruits remained within the inflorescence. Time of collection was critical for obtaining viable seeds, with successful harvesting limited to a short period (weeks) after maturation. The timing of flowering and fruit maturation were fairly consistent between species, populations and years in our study area. In L. concavum fruit production was increased in cultivation compared with wild populations. In all three species, very little or no germination of fruits occurred under nursery conditions. In vitro culture initiation was attempted using intact fruits, nicked fruits and seeds on 1/2MS (Murashige and Skoog) medium with 1 μM zeatin and 0.5M gibberellic acid in darkness. Culture of intact fruit resulted in no germination, while nicked fruit showed some germination response. Best results were achieved from seeds with germination occurring as early as 7 to 18 days depending on the species. Germination of L. concavum, L. laterale and L. longitudinale was 86%, 64% and 83% respectively within 5 weeks. Germination response was strongly influenced by seed maturity. Mature seeds germinated significantly faster than immature seeds. On a small proportion of cultured seeds, calli formed and differentiated into numerous plantlets on growth regulator-free medium. Given the promising results observed in this study, in vitro culture appears to be a practical means of mass propagating Lepidosperma species. © 2010 CSIRO.

    AB - Field studies of fruit production from Lepidosperma concavum R.Br., L. laterale R.Br. and L. longitudinale Labill. showed that large proportions (21-77%) of fruits were unfilled and that filled and unfilled fruits looked alike. Bagging of inflorescences demonstrated that filled fruits tended to be shed, while empty fruits remained within the inflorescence. Time of collection was critical for obtaining viable seeds, with successful harvesting limited to a short period (weeks) after maturation. The timing of flowering and fruit maturation were fairly consistent between species, populations and years in our study area. In L. concavum fruit production was increased in cultivation compared with wild populations. In all three species, very little or no germination of fruits occurred under nursery conditions. In vitro culture initiation was attempted using intact fruits, nicked fruits and seeds on 1/2MS (Murashige and Skoog) medium with 1 μM zeatin and 0.5M gibberellic acid in darkness. Culture of intact fruit resulted in no germination, while nicked fruit showed some germination response. Best results were achieved from seeds with germination occurring as early as 7 to 18 days depending on the species. Germination of L. concavum, L. laterale and L. longitudinale was 86%, 64% and 83% respectively within 5 weeks. Germination response was strongly influenced by seed maturity. Mature seeds germinated significantly faster than immature seeds. On a small proportion of cultured seeds, calli formed and differentiated into numerous plantlets on growth regulator-free medium. Given the promising results observed in this study, in vitro culture appears to be a practical means of mass propagating Lepidosperma species. © 2010 CSIRO.

    U2 - 10.1071/BT09183

    DO - 10.1071/BT09183

    M3 - Article

    VL - 58

    SP - 107

    EP - 114

    JO - Australian Journal of Botany

    JF - Australian Journal of Botany

    SN - 0067-1924

    IS - 2

    ER -