In Phase HLA Genotyping by Next Generation Sequencing — A Comparison Between Two Massively Parallel Sequencing Bench-Top Systems, the Roche GS Junior and Ion Torrent PGM

Jerzy Kulski, Shingo Suzuki, Y. Ozaki, S. Mitsunaga, Hidetoshi Inoko, Takashi Shiina

    Research output: Chapter in Book/Conference paperChapterpeer-review

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    Abstract

    We have developed procedures for massively parallel sequencing of multiplex products that can be used for several benchtop sequencing platforms. We have obtained sequences of sufficient high quality to permit accurate HLA in phase genotypes across the full-length gene from the 5’ promoter/enhancer region to the 3’ UTR for most of the classical class I and class II genes. The use of sample tags or barcodes allows for optimization of second generation sequencing technologies by pooling samples and sequencing multiple samples in parallel for time- and cost-efficient workflows. We are currently working towards optimizing the Ion PGM/SS-SBT method for HLA in phase genotyping for both the clinical diagnostic and research laboratories.
    Original languageEnglish
    Title of host publicationHLA and Associated Important Diseases
    EditorsYongzhi Xi
    Place of PublicationCroatia
    PublisherInTech
    Pages141-181
    Number of pages41
    ISBN (Print)978-953-51-1230-3
    DOIs
    Publication statusPublished - 19 Mar 2014

    Publication series

    NameImmunology, Allergology and Rheumatology

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