Abstract
In plant mitochondria, the 5′ ends of many transcripts are generated post-transcriptionally. We show that the pentatricopeptide repeat (PPR) protein RNA PROCESSING FACTOR 4 (RPF4) supports the generation of extra 5′ ends of ccmB transcripts in Landsberg erecta (Ler) and a number of other Arabidopsis thaliana ecotypes. RPF4 was identified in Ler applying a forward genetic approach supported by complementation studies of ecotype Columbia (Col), which generates the Ler-type extra ccmB 5′ termini only after the introduction of the RPF4 allele from Ler. Studies with chimeric RPF4 proteins composed of various parts of the RPF4 proteins from Ler and Col identified differences in the N-terminal and central PPR motifs that explain ecotype-specific variations in ccmB processing. These results fit well with binding site predictions in ccmB transcripts based on the known determinants of nucleotide base recognition by PPR motifs.
| Original language | English |
|---|---|
| Pages (from-to) | 659-668 |
| Journal | Plant Molecular Biology |
| Volume | 93 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Apr 2017 |
Fingerprint
Cite this
}
In Arabidopsis thaliana distinct alleles encoding mitochondrial RNA PROCESSING FACTOR 4 support the generation of additional 5′ termini of ccmB transcripts. / Stoll, Katrin; Jonietz, Christian; Schleicher, Sarah; des Francs-Small, Catherine Colas; Small, Ian; Binder, Stefan.
In: Plant Molecular Biology, Vol. 93, No. 6, 04.2017, p. 659-668.Research output: Contribution to journal › Article
TY - JOUR
T1 - In Arabidopsis thaliana distinct alleles encoding mitochondrial RNA PROCESSING FACTOR 4 support the generation of additional 5′ termini of ccmB transcripts
AU - Stoll, Katrin
AU - Jonietz, Christian
AU - Schleicher, Sarah
AU - des Francs-Small, Catherine Colas
AU - Small, Ian
AU - Binder, Stefan
PY - 2017/4
Y1 - 2017/4
N2 - In plant mitochondria, the 5′ ends of many transcripts are generated post-transcriptionally. We show that the pentatricopeptide repeat (PPR) protein RNA PROCESSING FACTOR 4 (RPF4) supports the generation of extra 5′ ends of ccmB transcripts in Landsberg erecta (Ler) and a number of other Arabidopsis thaliana ecotypes. RPF4 was identified in Ler applying a forward genetic approach supported by complementation studies of ecotype Columbia (Col), which generates the Ler-type extra ccmB 5′ termini only after the introduction of the RPF4 allele from Ler. Studies with chimeric RPF4 proteins composed of various parts of the RPF4 proteins from Ler and Col identified differences in the N-terminal and central PPR motifs that explain ecotype-specific variations in ccmB processing. These results fit well with binding site predictions in ccmB transcripts based on the known determinants of nucleotide base recognition by PPR motifs.
AB - In plant mitochondria, the 5′ ends of many transcripts are generated post-transcriptionally. We show that the pentatricopeptide repeat (PPR) protein RNA PROCESSING FACTOR 4 (RPF4) supports the generation of extra 5′ ends of ccmB transcripts in Landsberg erecta (Ler) and a number of other Arabidopsis thaliana ecotypes. RPF4 was identified in Ler applying a forward genetic approach supported by complementation studies of ecotype Columbia (Col), which generates the Ler-type extra ccmB 5′ termini only after the introduction of the RPF4 allele from Ler. Studies with chimeric RPF4 proteins composed of various parts of the RPF4 proteins from Ler and Col identified differences in the N-terminal and central PPR motifs that explain ecotype-specific variations in ccmB processing. These results fit well with binding site predictions in ccmB transcripts based on the known determinants of nucleotide base recognition by PPR motifs.
KW - 5′ end polymorphisms
KW - Arabidopsis thaliana
KW - Mitochondria
KW - Pentatricopeptide repeat proteins
KW - RNA processing
UR - http://www.scopus.com/inward/record.url?scp=85013391417&partnerID=8YFLogxK
U2 - 10.1007/s11103-017-0591-y
DO - 10.1007/s11103-017-0591-y
M3 - Article
VL - 93
SP - 659
EP - 668
JO - Plant Molecular Biology
JF - Plant Molecular Biology
SN - 0167-4412
IS - 6
ER -