Impurity of recombinant adeno-associated virus type 2 affects the transduction characteristics following a subretinal injection in the rat

W-Y. Shen; Y.K.Y. Lai; Chooi-May Lai; Elizabeth Rakoczy

doi:10.1016/j.visres.2003.09.032

Impurity of recombinant adeno-associated virus type 2 affects the transduction characteristics following a subretinal injection in the rat

W-Y. Shen, Y.K.Y. Lai, Chooi-May Lai, Elizabeth Rakoczy

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6 Citations (Scopus)

Abstract

We recently reported that different purification methods of recombinant adeno-associated virus type 2 (rAAV2) affect the transduction characteristics following subretinal injection. In this study, we examined the roles of contaminant proteins from the HEK-293 cells and helper adenovirus, inactivation of helper adenovirus and cell stress induced by DNA-damaging agents in rAAV-mediated retinal transduction. Our results showed that contaminating factors/proteins resulting from the helper E1 deleted adenovirus are possibly responsible for efficient RPE transduction. Future studies of these factors will undoubtedly lead to development of new therapeutic approaches to PR- and RPE-specific retinal diseases. (C) 2003 Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	339-348
Journal	Vision Research
Volume	44
Issue number	4
DOIs	https://doi.org/10.1016/j.visres.2003.09.032
Publication status	Published - 2004

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title = "Impurity of recombinant adeno-associated virus type 2 affects the transduction characteristics following a subretinal injection in the rat",

abstract = "We recently reported that different purification methods of recombinant adeno-associated virus type 2 (rAAV2) affect the transduction characteristics following subretinal injection. In this study, we examined the roles of contaminant proteins from the HEK-293 cells and helper adenovirus, inactivation of helper adenovirus and cell stress induced by DNA-damaging agents in rAAV-mediated retinal transduction. Our results showed that contaminating factors/proteins resulting from the helper E1 deleted adenovirus are possibly responsible for efficient RPE transduction. Future studies of these factors will undoubtedly lead to development of new therapeutic approaches to PR- and RPE-specific retinal diseases. (C) 2003 Elsevier Ltd. All rights reserved.",

author = "W-Y. Shen and Y.K.Y. Lai and Chooi-May Lai and Elizabeth Rakoczy",

year = "2004",

doi = "10.1016/j.visres.2003.09.032",

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T1 - Impurity of recombinant adeno-associated virus type 2 affects the transduction characteristics following a subretinal injection in the rat

AU - Shen, W-Y.

AU - Lai, Y.K.Y.

AU - Lai, Chooi-May

AU - Rakoczy, Elizabeth

PY - 2004

Y1 - 2004

N2 - We recently reported that different purification methods of recombinant adeno-associated virus type 2 (rAAV2) affect the transduction characteristics following subretinal injection. In this study, we examined the roles of contaminant proteins from the HEK-293 cells and helper adenovirus, inactivation of helper adenovirus and cell stress induced by DNA-damaging agents in rAAV-mediated retinal transduction. Our results showed that contaminating factors/proteins resulting from the helper E1 deleted adenovirus are possibly responsible for efficient RPE transduction. Future studies of these factors will undoubtedly lead to development of new therapeutic approaches to PR- and RPE-specific retinal diseases. (C) 2003 Elsevier Ltd. All rights reserved.

AB - We recently reported that different purification methods of recombinant adeno-associated virus type 2 (rAAV2) affect the transduction characteristics following subretinal injection. In this study, we examined the roles of contaminant proteins from the HEK-293 cells and helper adenovirus, inactivation of helper adenovirus and cell stress induced by DNA-damaging agents in rAAV-mediated retinal transduction. Our results showed that contaminating factors/proteins resulting from the helper E1 deleted adenovirus are possibly responsible for efficient RPE transduction. Future studies of these factors will undoubtedly lead to development of new therapeutic approaches to PR- and RPE-specific retinal diseases. (C) 2003 Elsevier Ltd. All rights reserved.

U2 - 10.1016/j.visres.2003.09.032

DO - 10.1016/j.visres.2003.09.032

M3 - Article

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VL - 44

SP - 339

EP - 348

JO - Vision Research

JF - Vision Research

IS - 4

ER -

Impurity of recombinant adeno-associated virus type 2 affects the transduction characteristics following a subretinal injection in the rat

Abstract

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