Bacterial blight (BB), caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the most devastating diseases of rice world-wide; it is also a serious problem of hybrid rice production in China. In this study, a molecular marker-assisted introgression of Xa21, a gene highly resistant to a broad spectrum of Xoo strains, from 'IRBB21' was performed to improve the BE resistance of '6078', a new restorer line with high yielding potential. The entire process took one generation of crossing followed by three generations of backcrossing and one generation of selfing. The presence of Xa21 in each generation was determined by both polymerase chain reaction (PCR) and pathogen inoculation. Recombinations between Xa21 and flanking markers were identified by PCR analysis. Background selection was conducted in BC1F1 and BC2F1 using amplified fragment length polymorphism (AFLP) markers detecting a total of 129 polymorphic bands between '6078' and 'IRBB21'. The individual selected in BC3F2, or '6078'(Xa21), carried a fragment of less than 3.8 cM from the donor line in the Xa21 region on chromosome 11, and about 98.8% of the generic background from the recurrent parent. The results showed that '6078'(Xn21) had the same level and spectrum of BB resistance as the donor parent 'IRBB21', while maintaining the agronomic performance and combining ability of the original 6078. A significant increase in BE resistance was also achieved in the hybrid using 6078(Xa21) as the restorer line.
|Publication status||Published - 2001|