Improved pulsed field gel electrophoresis method for Moraxella catarrhalis

N.M. Pingault; Deborah Lehmann; Thomas Riley

doi:10.1016/j.mimet.2008.05.021

Improved pulsed field gel electrophoresis method for Moraxella catarrhalis

N.M. Pingault, Deborah Lehmann, Thomas Riley

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

An improved PFGE method for the molecular typing of Moraxella catarrhalis is described. A modified PulseNet method using higher concentrations of EDTA and proteinase K, together with increased reagent volumes and incubation temperatures resulted in improved results and a more rapid turnaround time compared to PFGE methods currently used. (C) 2008 Elsevier BY. All rights reserved.

Original language	English
Pages (from-to)	344-345
Journal	Journal of Microbiological Methods
Volume	75
Issue number	2
DOIs	https://doi.org/10.1016/j.mimet.2008.05.021
Publication status	Published - 2008

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10.1016/j.mimet.2008.05.021

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title = "Improved pulsed field gel electrophoresis method for Moraxella catarrhalis",

abstract = "An improved PFGE method for the molecular typing of Moraxella catarrhalis is described. A modified PulseNet method using higher concentrations of EDTA and proteinase K, together with increased reagent volumes and incubation temperatures resulted in improved results and a more rapid turnaround time compared to PFGE methods currently used. (C) 2008 Elsevier BY. All rights reserved.",

author = "N.M. Pingault and Deborah Lehmann and Thomas Riley",

year = "2008",

doi = "10.1016/j.mimet.2008.05.021",

language = "English",

volume = "75",

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journal = "Journal of Microbiological Methods",

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T1 - Improved pulsed field gel electrophoresis method for Moraxella catarrhalis

AU - Pingault, N.M.

AU - Lehmann, Deborah

AU - Riley, Thomas

PY - 2008

Y1 - 2008

N2 - An improved PFGE method for the molecular typing of Moraxella catarrhalis is described. A modified PulseNet method using higher concentrations of EDTA and proteinase K, together with increased reagent volumes and incubation temperatures resulted in improved results and a more rapid turnaround time compared to PFGE methods currently used. (C) 2008 Elsevier BY. All rights reserved.

AB - An improved PFGE method for the molecular typing of Moraxella catarrhalis is described. A modified PulseNet method using higher concentrations of EDTA and proteinase K, together with increased reagent volumes and incubation temperatures resulted in improved results and a more rapid turnaround time compared to PFGE methods currently used. (C) 2008 Elsevier BY. All rights reserved.

U2 - 10.1016/j.mimet.2008.05.021

DO - 10.1016/j.mimet.2008.05.021

M3 - Article

C2 - 18586342

SN - 0167-7012

VL - 75

SP - 344

EP - 345

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 2

ER -

Improved pulsed field gel electrophoresis method for Moraxella catarrhalis

Abstract

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