Improved Biological Properties of Calcium Phosphate Cement by Nacre Incorporation: An In Vitro Study

Rui Ruan, Minghao Zheng, Junjie Gao, Euphemie Landao-Bassonga, Lianzhi Chen, Peilin Chen, Tao Wang, Xueling Zhao

Research output: Contribution to journalArticle

Abstract

To investigate the effect of incorporating nacre into calcium phosphate cement (CPC), one of the most commonly used bone substitutes. In this study, nacre at 25 wt% and 50 wt% was incorporated into a CPC powder mixture. The composites were characterized, and their in vitro osteoblast functions were examined. X-ray diffraction (XRD) patterns revealed that the inorganic composition of the composites mainly was consisted of aragonite, hydroxyapatite ( HA) and carbonate-apatite. Scanning electron microscopy (SEM) and micro-computed tomography (micro-CT) images indicated that the porosity of the CPC composites was increased by nacre incorporation. However, the setting time increased, and the compressive strength decreased with increased nacre addition into CPC. In addition, the in vitro degradation rate of the nacre/CPC composites was faster than that of CPC. Cell tests revealed that nacre/CPC composites are cytocompatible and, compared with CPC, could enhance the cell adhesion, spreading and proliferation of MC3T3-E1 cells on materials. Moreover, the composites exhibited stronger osteogenicity than CPC and inorganic calcium carbonate-incorporated CPC composites by promoting alkaline phosphatase ( ALP) activity and the gene expression levels of ALP, runt-related transcription factor 2 (Runx2), osteocalcin (OCN) and osteopontin (OPN). Thus, nacre could be a promising additive to increase the biological performance of CPC.

Original languageEnglish
Pages (from-to)67-79
Number of pages13
JournalJournal of Biomaterials and Tissue Engineering
Volume8
Issue number1
DOIs
Publication statusPublished - Jan 2018

Cite this

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title = "Improved Biological Properties of Calcium Phosphate Cement by Nacre Incorporation: An In Vitro Study",
abstract = "To investigate the effect of incorporating nacre into calcium phosphate cement (CPC), one of the most commonly used bone substitutes. In this study, nacre at 25 wt{\%} and 50 wt{\%} was incorporated into a CPC powder mixture. The composites were characterized, and their in vitro osteoblast functions were examined. X-ray diffraction (XRD) patterns revealed that the inorganic composition of the composites mainly was consisted of aragonite, hydroxyapatite ( HA) and carbonate-apatite. Scanning electron microscopy (SEM) and micro-computed tomography (micro-CT) images indicated that the porosity of the CPC composites was increased by nacre incorporation. However, the setting time increased, and the compressive strength decreased with increased nacre addition into CPC. In addition, the in vitro degradation rate of the nacre/CPC composites was faster than that of CPC. Cell tests revealed that nacre/CPC composites are cytocompatible and, compared with CPC, could enhance the cell adhesion, spreading and proliferation of MC3T3-E1 cells on materials. Moreover, the composites exhibited stronger osteogenicity than CPC and inorganic calcium carbonate-incorporated CPC composites by promoting alkaline phosphatase ( ALP) activity and the gene expression levels of ALP, runt-related transcription factor 2 (Runx2), osteocalcin (OCN) and osteopontin (OPN). Thus, nacre could be a promising additive to increase the biological performance of CPC.",
keywords = "Nacre, Calcium Phosphate Cement, In Vitro, Osteoinductivity, Porosity, BONE SUBSTITUTE MATERIALS, MESENCHYMAL STEM-CELLS, DEGRADATION PATTERN, PEARL, COMPOSITE, HYDROXYAPATITE, REGENERATION, SCAFFOLDS, CARBONATE, STIMULATION",
author = "Rui Ruan and Minghao Zheng and Junjie Gao and Euphemie Landao-Bassonga and Lianzhi Chen and Peilin Chen and Tao Wang and Xueling Zhao",
year = "2018",
month = "1",
doi = "10.1166/jbt.2018.1720",
language = "English",
volume = "8",
pages = "67--79",
journal = "Journal of Biomaterials and Tissue Engineering",
issn = "2157-9083",
publisher = "American Scientific Publishers",
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Improved Biological Properties of Calcium Phosphate Cement by Nacre Incorporation : An In Vitro Study. / Ruan, Rui; Zheng, Minghao; Gao, Junjie; Landao-Bassonga, Euphemie; Chen, Lianzhi; Chen, Peilin; Wang, Tao; Zhao, Xueling.

In: Journal of Biomaterials and Tissue Engineering, Vol. 8, No. 1, 01.2018, p. 67-79.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Improved Biological Properties of Calcium Phosphate Cement by Nacre Incorporation

T2 - An In Vitro Study

AU - Ruan, Rui

AU - Zheng, Minghao

AU - Gao, Junjie

AU - Landao-Bassonga, Euphemie

AU - Chen, Lianzhi

AU - Chen, Peilin

AU - Wang, Tao

AU - Zhao, Xueling

PY - 2018/1

Y1 - 2018/1

N2 - To investigate the effect of incorporating nacre into calcium phosphate cement (CPC), one of the most commonly used bone substitutes. In this study, nacre at 25 wt% and 50 wt% was incorporated into a CPC powder mixture. The composites were characterized, and their in vitro osteoblast functions were examined. X-ray diffraction (XRD) patterns revealed that the inorganic composition of the composites mainly was consisted of aragonite, hydroxyapatite ( HA) and carbonate-apatite. Scanning electron microscopy (SEM) and micro-computed tomography (micro-CT) images indicated that the porosity of the CPC composites was increased by nacre incorporation. However, the setting time increased, and the compressive strength decreased with increased nacre addition into CPC. In addition, the in vitro degradation rate of the nacre/CPC composites was faster than that of CPC. Cell tests revealed that nacre/CPC composites are cytocompatible and, compared with CPC, could enhance the cell adhesion, spreading and proliferation of MC3T3-E1 cells on materials. Moreover, the composites exhibited stronger osteogenicity than CPC and inorganic calcium carbonate-incorporated CPC composites by promoting alkaline phosphatase ( ALP) activity and the gene expression levels of ALP, runt-related transcription factor 2 (Runx2), osteocalcin (OCN) and osteopontin (OPN). Thus, nacre could be a promising additive to increase the biological performance of CPC.

AB - To investigate the effect of incorporating nacre into calcium phosphate cement (CPC), one of the most commonly used bone substitutes. In this study, nacre at 25 wt% and 50 wt% was incorporated into a CPC powder mixture. The composites were characterized, and their in vitro osteoblast functions were examined. X-ray diffraction (XRD) patterns revealed that the inorganic composition of the composites mainly was consisted of aragonite, hydroxyapatite ( HA) and carbonate-apatite. Scanning electron microscopy (SEM) and micro-computed tomography (micro-CT) images indicated that the porosity of the CPC composites was increased by nacre incorporation. However, the setting time increased, and the compressive strength decreased with increased nacre addition into CPC. In addition, the in vitro degradation rate of the nacre/CPC composites was faster than that of CPC. Cell tests revealed that nacre/CPC composites are cytocompatible and, compared with CPC, could enhance the cell adhesion, spreading and proliferation of MC3T3-E1 cells on materials. Moreover, the composites exhibited stronger osteogenicity than CPC and inorganic calcium carbonate-incorporated CPC composites by promoting alkaline phosphatase ( ALP) activity and the gene expression levels of ALP, runt-related transcription factor 2 (Runx2), osteocalcin (OCN) and osteopontin (OPN). Thus, nacre could be a promising additive to increase the biological performance of CPC.

KW - Nacre

KW - Calcium Phosphate Cement

KW - In Vitro

KW - Osteoinductivity

KW - Porosity

KW - BONE SUBSTITUTE MATERIALS

KW - MESENCHYMAL STEM-CELLS

KW - DEGRADATION PATTERN

KW - PEARL

KW - COMPOSITE

KW - HYDROXYAPATITE

KW - REGENERATION

KW - SCAFFOLDS

KW - CARBONATE

KW - STIMULATION

U2 - 10.1166/jbt.2018.1720

DO - 10.1166/jbt.2018.1720

M3 - Article

VL - 8

SP - 67

EP - 79

JO - Journal of Biomaterials and Tissue Engineering

JF - Journal of Biomaterials and Tissue Engineering

SN - 2157-9083

IS - 1

ER -