Implantation and recording of wireless electroretinogram and visual evoked potential in conscious rats

Jason Charng, Zheng He, Bang Bui, Algis Vingrys, Magnus Ivarsson, Rebecca Fish, Rachel Gurrell, Christine Nguyen

Research output: Contribution to journalArticle

Abstract

The full-field electroretinogram (ERG) and visual evoked potential (VEP) are useful tools to assess retinal and visual pathway integrity in both laboratory and clinical settings. Currently, preclinical ERG and VEP measurements are performed with anesthesia to ensure stable electrode placements. However, the very presence of anesthesia has been shown to contaminate normal physiological responses. To overcome these anesthesia confounds, we develop a novel platform to assay ERG and VEP in conscious rats. Electrodes are surgically implanted subconjunctivally on the eye to assay the ERG and epidurally over the visual cortex to measure the VEP. A range of amplitude and sensitivity/ timing parameters are assayed for both the ERG and VEP at increasing luminous energies. The ERG and VEP signals are shown to be stable and repeatable for at least 4 weeks post surgical implantation. This ability to record ERG and VEP signals without anesthesia confounds in the preclinical setting should provide superior translation to clinical data.

Original languageEnglish
Article numbere54160
Number of pages6
JournalJOURNAL OF VISUALIZED EXPERIMENTS : JOVE
Volume2016
Issue number112
DOIs
Publication statusPublished - 29 Jun 2016
Externally publishedYes

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