Projects per year
BACKGROUND: Late-onset sepsis (LOS) with Staphylococcus epidermidis is common in preterm infants, but the immunological mechanisms underlying heightened susceptibility are poorly understood.
AIM: To characterise the ontogeny of cytokine responses to live S. epidermidis in preterm infants with and without subsequent Gram-positive LOS.
METHODS: A prospective observational cohort study of preterm infants (<30 weeks gestational age, GA) with blood sampling on days 1, 7, 14, 21 and 28 of life. Cytokine responses in peripheral whole blood stimulated with live S. epidermidis were analysed by 11-plex immunoassay.
RESULTS: Of 129 infants (mean GA 26.2 weeks, mean BW 887g), 23 (17.8%) had confirmed LOS with Gram-positive organisms and 15 (11.6%) infants had clinical sepsis, with median onset at 13 and 15 days, respectively.
CONCLUSIONS: Cytokine responses to live S. epidermidis challenge are impaired in infants with LOS and precede the onset of clinical illness. Quantifying pathogen-specific cytokine responses at day 7 may identify high risk preterm infants at greatest risk of LOS and prospective replication is warranted.
|Journal||Clinical infectious diseases : an official publication of the Infectious Diseases Society of America|
|Publication status||E-pub ahead of print - 21 Jan 2020|
A Prospective Study of the Development of Innate Immunity in Preterm Infants and Susceptability to Neonatal Infection
31/12/08 → 31/12/11