Impaired cytokine responses to live Staphylococcus epidermidis in preterm infants precede Gram-positive late-onset sepsis

Tobias Strunk; Julie Hibbert; Dorota Doherty; Elizabeth Nathan; Karen Simmer; Peter Richmond; Andrew Currie; David Burgner

doi:10.1093/cid/ciaa063

Impaired cytokine responses to live Staphylococcus epidermidis in preterm infants precede Gram-positive late-onset sepsis

Tobias Strunk, Julie Hibbert, Dorota Doherty, Elizabeth Nathan, Karen Simmer, Peter Richmond, Andrew Currie, David Burgner

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

BACKGROUND: Late-onset sepsis (LOS) with Staphylococcus epidermidis is common in preterm infants, but the immunological mechanisms underlying heightened susceptibility are poorly understood.

AIM: To characterise the ontogeny of cytokine responses to live S. epidermidis in preterm infants with and without subsequent Gram-positive LOS.

METHODS: A prospective observational cohort study of preterm infants (<30 weeks gestational age, GA) with blood sampling on days 1, 7, 14, 21 and 28 of life. Cytokine responses in peripheral whole blood stimulated with live S. epidermidis were analysed by 11-plex immunoassay.

RESULTS: Of 129 infants (mean GA 26.2 weeks, mean BW 887g), 23 (17.8%) had confirmed LOS with Gram-positive organisms and 15 (11.6%) infants had clinical sepsis, with median onset at 13 and 15 days, respectively.

CONCLUSIONS: Cytokine responses to live S. epidermidis challenge are impaired in infants with LOS and precede the onset of clinical illness. Quantifying pathogen-specific cytokine responses at day 7 may identify high risk preterm infants at greatest risk of LOS and prospective replication is warranted.

Original language	English
Pages (from-to)	271-278
Number of pages	8
Journal	Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
Volume	72
Issue number	2
Early online date	21 Jan 2020
DOIs	https://doi.org/10.1093/cid/ciaa063
Publication status	Published - 15 Jan 2021

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10.1093/cid/ciaa063

A Prospective Study of the Development of Innate Immunity in Preterm Infants and Susceptability to Neonatal Infection
Burgner, D. (Investigator 01), Currie, A. (Investigator 02), Richmond, P. (Investigator 03), Simmer, K. (Investigator 04), Levy, O. (Investigator 05) & Strunk, T. (Investigator 06)
NHMRC National Health and Medical Research Council
31/12/08 → 31/12/11
Project: Research

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Strunk, T., Hibbert, J., Doherty, D., Nathan, E., Simmer, K., Richmond, P., Currie, A., & Burgner, D. (2021). Impaired cytokine responses to live Staphylococcus epidermidis in preterm infants precede Gram-positive late-onset sepsis. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 72(2), 271-278. https://doi.org/10.1093/cid/ciaa063

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title = "Impaired cytokine responses to live Staphylococcus epidermidis in preterm infants precede Gram-positive late-onset sepsis",

abstract = "BACKGROUND: Late-onset sepsis (LOS) with Staphylococcus epidermidis is common in preterm infants, but the immunological mechanisms underlying heightened susceptibility are poorly understood.AIM: To characterise the ontogeny of cytokine responses to live S. epidermidis in preterm infants with and without subsequent Gram-positive LOS.METHODS: A prospective observational cohort study of preterm infants (<30 weeks gestational age, GA) with blood sampling on days 1, 7, 14, 21 and 28 of life. Cytokine responses in peripheral whole blood stimulated with live S. epidermidis were analysed by 11-plex immunoassay.RESULTS: Of 129 infants (mean GA 26.2 weeks, mean BW 887g), 23 (17.8%) had confirmed LOS with Gram-positive organisms and 15 (11.6%) infants had clinical sepsis, with median onset at 13 and 15 days, respectively.CONCLUSIONS: Cytokine responses to live S. epidermidis challenge are impaired in infants with LOS and precede the onset of clinical illness. Quantifying pathogen-specific cytokine responses at day 7 may identify high risk preterm infants at greatest risk of LOS and prospective replication is warranted.",

author = "Tobias Strunk and Julie Hibbert and Dorota Doherty and Elizabeth Nathan and Karen Simmer and Peter Richmond and Andrew Currie and David Burgner",

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language = "English",

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Strunk, T , Hibbert, J , Doherty, D , Nathan, E , Simmer, K , Richmond, P, Currie, A & Burgner, D 2021, 'Impaired cytokine responses to live Staphylococcus epidermidis in preterm infants precede Gram-positive late-onset sepsis', Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, vol. 72, no. 2, pp. 271-278. https://doi.org/10.1093/cid/ciaa063

Impaired cytokine responses to live Staphylococcus epidermidis in preterm infants precede Gram-positive late-onset sepsis. / Strunk, Tobias ; Hibbert, Julie ; Doherty, Dorota et al.
In: Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, Vol. 72, No. 2, 15.01.2021, p. 271-278.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Impaired cytokine responses to live Staphylococcus epidermidis in preterm infants precede Gram-positive late-onset sepsis

AU - Strunk, Tobias

AU - Hibbert, Julie

AU - Doherty, Dorota

AU - Nathan, Elizabeth

AU - Simmer, Karen

AU - Richmond, Peter

AU - Currie, Andrew

AU - Burgner, David

PY - 2021/1/15

Y1 - 2021/1/15

N2 - BACKGROUND: Late-onset sepsis (LOS) with Staphylococcus epidermidis is common in preterm infants, but the immunological mechanisms underlying heightened susceptibility are poorly understood.AIM: To characterise the ontogeny of cytokine responses to live S. epidermidis in preterm infants with and without subsequent Gram-positive LOS.METHODS: A prospective observational cohort study of preterm infants (<30 weeks gestational age, GA) with blood sampling on days 1, 7, 14, 21 and 28 of life. Cytokine responses in peripheral whole blood stimulated with live S. epidermidis were analysed by 11-plex immunoassay.RESULTS: Of 129 infants (mean GA 26.2 weeks, mean BW 887g), 23 (17.8%) had confirmed LOS with Gram-positive organisms and 15 (11.6%) infants had clinical sepsis, with median onset at 13 and 15 days, respectively.CONCLUSIONS: Cytokine responses to live S. epidermidis challenge are impaired in infants with LOS and precede the onset of clinical illness. Quantifying pathogen-specific cytokine responses at day 7 may identify high risk preterm infants at greatest risk of LOS and prospective replication is warranted.

AB - BACKGROUND: Late-onset sepsis (LOS) with Staphylococcus epidermidis is common in preterm infants, but the immunological mechanisms underlying heightened susceptibility are poorly understood.AIM: To characterise the ontogeny of cytokine responses to live S. epidermidis in preterm infants with and without subsequent Gram-positive LOS.METHODS: A prospective observational cohort study of preterm infants (<30 weeks gestational age, GA) with blood sampling on days 1, 7, 14, 21 and 28 of life. Cytokine responses in peripheral whole blood stimulated with live S. epidermidis were analysed by 11-plex immunoassay.RESULTS: Of 129 infants (mean GA 26.2 weeks, mean BW 887g), 23 (17.8%) had confirmed LOS with Gram-positive organisms and 15 (11.6%) infants had clinical sepsis, with median onset at 13 and 15 days, respectively.CONCLUSIONS: Cytokine responses to live S. epidermidis challenge are impaired in infants with LOS and precede the onset of clinical illness. Quantifying pathogen-specific cytokine responses at day 7 may identify high risk preterm infants at greatest risk of LOS and prospective replication is warranted.

U2 - 10.1093/cid/ciaa063

DO - 10.1093/cid/ciaa063

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C2 - 31960030

SN - 1058-4838

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JO - Clinical infectious diseases : an official publication of the Infectious Diseases Society of America

JF - Clinical infectious diseases : an official publication of the Infectious Diseases Society of America

IS - 2

ER -

Impaired cytokine responses to live Staphylococcus epidermidis in preterm infants precede Gram-positive late-onset sepsis

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A Prospective Study of the Development of Innate Immunity in Preterm Infants and Susceptability to Neonatal Infection

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