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Abstract
AIM: To investigate the impact of expression mode: electric breast pump or hand expression, and timing of sample collection: pre- and post-milk ejection on human milk bacterial DNA profiles.
METHODS AND RESULTS: Three human milk samples from the same breast were collected from 30 breastfeeding mothers: a pre-milk ejection pump-expressed sample (pre-pump), a post-milk ejection pump-expressed sample (post-pump), and a post-milk ejection hand-expressed sample (post-hand). Full-length 16S rRNA gene sequencing was used to assess milk bacterial DNA profiles. Bacterial profiles did not differ significantly based on mode of expression nor timing of sample collection. No significant differences were detected in the relative abundance of any OTUs based on expression condition (pre-pump/ post-pump and post-pump/post-hand) with univariate linear mixed-effects regression analyses (all p-values >0.01; α = 0.01). Similarly, no difference in richness was observed between sample types (number of observed OTUs: post-pump/post-hand P = 0.13; pre-pump/post-pump P = 0. 45).
CONCLUSION: Bacterial DNA profiles of human milk did not differ according to either expression method or timing of sample collection.
SIGNIFICANCE AND IMPACT OF STUDY: Hand or pump expression can be utilized to collect samples for microbiome studies. This has implications for the design of future human milk microbiome studies.
Original language | English |
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Pages (from-to) | 988-995 |
Number of pages | 8 |
Journal | Journal of Applied Microbiology |
Volume | 131 |
Issue number | 2 |
Early online date | 9 Jan 2021 |
DOIs | |
Publication status | Published - Aug 2021 |
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Dive into the research topics of 'Impact of expression mode and timing of sample collection, relative to milk ejection, on human milk bacterial DNA profiles'. Together they form a unique fingerprint.Projects
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A prospective, open-label, single-centre/multi-site, randomized clinical trial of a novel maternal microbiological “screen & treat” program compared with normal care for the prevention of preterm birth
Newnham, J. (Investigator 01), Keelan, J. (Investigator 02), Payne, M. (Investigator 03) & Doherty, D. (Investigator 04)
NHMRC National Health and Medical Research Council
1/01/18 → 31/12/21
Project: Research