© 2016Crop rotation and tillage systems have important implications for weed infestation and crop productivity. In this study, five tillage systems viz. zero tillage (ZT), conventional tillage (CT), deep tillage (DT), bed sowing (60/30 cm with four rows; BS1) and bed sowing (90/45 cm with six rows; BS2) were evaluated in five different crop rotations viz. fallow-wheat (FW), rice-wheat (RW), cotton-wheat (CW), mungbean-wheat (MW) and sorghum-wheat (SW) for their effect on weed infestation and productivity of bread wheat. Interaction between different tillage practices and cropping systems had significant effect on density and dry biomass of total, broadleaved and grass weeds, agronomic and yield-related traits, and grain yield of bread wheat. The un-disturbed soils (ZT) under fallow-wheat or mungbean-wheat rotations favoured the weed prevalence (a total weed dry biomass of 72.4–109.6 and 105.6–112.1 g m−2 in first and second year, respectively). Contrary to this, the disturbed soils (CT, DT, BS1 and BS2) had less weed infestation with either of the rotations (a total weed biomass of 0.4–7.1 and 1.1–5.4 g m−2 in first and second year, respectively). Sorghum-wheat rotation had strong suppressive effect on weed infestation in all tillage systems. The impact of crop rotation was more visible during second year of experimentation. Bed sown wheat (BS1 and BS2) in mungbean-wheat rotation had the highest wheat grain yield (6.30–6.47 t ha−1) compared to other tillage systems in different crop rotation combinations.