TY - JOUR
T1 - Immunocytologic dissection of the urine sediment using monoclonal antibodies
AU - Segasothy, M.
AU - Lau, T. M.
AU - Birch, D. F.
AU - Fairley, K. F.
AU - Kincaid-Smith, P.
PY - 1988
Y1 - 1988
N2 - A method to identify nucleated nonsquamous cells in urine using monoclonal antibodies and immunoperoxidase stain is described. Cells from washed deposits of midstream urine samples were transferred to gelatinized slides in a cytocentrifuge, air-dried, aceton fixed, and subjected to microwave irradiation. Slide preparations were then treated with monoclonal antibodies with the use of a four-layer peroxidase-antiperoxidase technique. It was possible to identify granulocytes, monocytes, lymphocytes, and renal epithelial and urothelial cells. This method was found to be helpful in determining the profiles of cells in urine in acute tubular necrosis, drug-related acute interstitial nephritis, and crescentic glomerulonephritis.
AB - A method to identify nucleated nonsquamous cells in urine using monoclonal antibodies and immunoperoxidase stain is described. Cells from washed deposits of midstream urine samples were transferred to gelatinized slides in a cytocentrifuge, air-dried, aceton fixed, and subjected to microwave irradiation. Slide preparations were then treated with monoclonal antibodies with the use of a four-layer peroxidase-antiperoxidase technique. It was possible to identify granulocytes, monocytes, lymphocytes, and renal epithelial and urothelial cells. This method was found to be helpful in determining the profiles of cells in urine in acute tubular necrosis, drug-related acute interstitial nephritis, and crescentic glomerulonephritis.
UR - http://www.scopus.com/inward/record.url?scp=0024259863&partnerID=8YFLogxK
U2 - 10.1093/ajcp/90.6.691
DO - 10.1093/ajcp/90.6.691
M3 - Article
C2 - 3057861
AN - SCOPUS:0024259863
SN - 0002-9173
VL - 90
SP - 691
EP - 696
JO - American Journal of Clinical Pathology
JF - American Journal of Clinical Pathology
IS - 6
ER -