Immunocytochemical Detection of Endothelin Receptors in Rat Cultured Airway Nerves

Lynette Fernandes, Peter Henry, L.J. Spalding, S.H. Cody, C.J. Pudney, Roy Goldie

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Endothelin-1 (ET-1) has been shown to potentiate cholinergic neurotransmission in human bronchus as well as in airways from a variety of animal species, suggesting that ET receptors exist prejunctionally on airway cholinergic nerves. We have successfully isolated and maintained rat tracheal parasympathetic neurons in culture. Most cultured cells were associated with specific fluorescence for the nerve cell marker protein gene product 9.5 (PGP 9.5). These cultures contained a high proportion of parasympathetic neurons. Importantly, specific immunofluorescent antibodies for ETB receptors were colocalized with those for PGP 9.5, Therefore, for the first time, ETB receptors have been shown to exist on airway parasympathetic neurons in culture.
Original languageEnglish
Pages (from-to)S222-S224
JournalJournal of Cardiovascular Pharmacology
Volume31 (Suppl 1)
DOIs
Publication statusPublished - 1998

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Endothelin Receptors
Neurons
Cholinergic Agents
Endothelin-1
Bronchi
Synaptic Transmission
Cultured Cells
Proteins
Fluorescence
Antibodies

Cite this

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title = "Immunocytochemical Detection of Endothelin Receptors in Rat Cultured Airway Nerves",
abstract = "Endothelin-1 (ET-1) has been shown to potentiate cholinergic neurotransmission in human bronchus as well as in airways from a variety of animal species, suggesting that ET receptors exist prejunctionally on airway cholinergic nerves. We have successfully isolated and maintained rat tracheal parasympathetic neurons in culture. Most cultured cells were associated with specific fluorescence for the nerve cell marker protein gene product 9.5 (PGP 9.5). These cultures contained a high proportion of parasympathetic neurons. Importantly, specific immunofluorescent antibodies for ETB receptors were colocalized with those for PGP 9.5, Therefore, for the first time, ETB receptors have been shown to exist on airway parasympathetic neurons in culture.",
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Immunocytochemical Detection of Endothelin Receptors in Rat Cultured Airway Nerves. / Fernandes, Lynette; Henry, Peter; Spalding, L.J.; Cody, S.H.; Pudney, C.J.; Goldie, Roy.

In: Journal of Cardiovascular Pharmacology, Vol. 31 (Suppl 1), 1998, p. S222-S224.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Immunocytochemical Detection of Endothelin Receptors in Rat Cultured Airway Nerves

AU - Fernandes, Lynette

AU - Henry, Peter

AU - Spalding, L.J.

AU - Cody, S.H.

AU - Pudney, C.J.

AU - Goldie, Roy

PY - 1998

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AB - Endothelin-1 (ET-1) has been shown to potentiate cholinergic neurotransmission in human bronchus as well as in airways from a variety of animal species, suggesting that ET receptors exist prejunctionally on airway cholinergic nerves. We have successfully isolated and maintained rat tracheal parasympathetic neurons in culture. Most cultured cells were associated with specific fluorescence for the nerve cell marker protein gene product 9.5 (PGP 9.5). These cultures contained a high proportion of parasympathetic neurons. Importantly, specific immunofluorescent antibodies for ETB receptors were colocalized with those for PGP 9.5, Therefore, for the first time, ETB receptors have been shown to exist on airway parasympathetic neurons in culture.

U2 - 10.1097/00005344-199800001-00062

DO - 10.1097/00005344-199800001-00062

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SP - S222-S224

JO - Journal of Cardiovascular Pharmacology

JF - Journal of Cardiovascular Pharmacology

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