Imaging Intracellular Metabolic Activity of Mammalian Oocytes by Dynamic Full-field Optical Coherence Microscopy

Seweryn Morawiec; Patrycjusz Stremplewski; Anna Ajduk; Brendan Kennedy; Maciej Szkulmowski

doi:10.1109/CLEO/EUROPE-EQEC57999.2023.10232362

Imaging Intracellular Metabolic Activity of Mammalian Oocytes by Dynamic Full-field Optical Coherence Microscopy

Seweryn Morawiec, Patrycjusz Stremplewski, Anna Ajduk, Brendan Kennedy, Maciej Szkulmowski

Electrical, Electronic and Computer Engineering

Research output: Chapter in Book/Conference paper › Conference paper › peer-review

Abstract

Full-field optical coherence microscopy (FF-OCM) is ideally suited to study living cells as it provides high-resolution cross-section images with no need for fluorescent labelling [1,2]. The OCM signal is proportional both to the scattering potential of the sample, providing structural information, and to the motion amplitude of the inter-cellular structures. Thus, the metabolic activity information can be extracted from the spatiotemporal analysis of the OCM images. This technique is particularly appealing for embryology to noninvasively assess the development potential of living cells, e.g., oocytes [3].

Original language	English
Title of host publication	2023 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference, CLEO/Europe-EQEC 2023
Place of Publication	USA
Publisher	IEEE, Institute of Electrical and Electronics Engineers
Number of pages	1
ISBN (Electronic)	9798350345995
DOIs	https://doi.org/10.1109/CLEO/EUROPE-EQEC57999.2023.10232362
Publication status	Published - 4 Sept 2023
Event	2023 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference - Munich, Germany Duration: 26 Jun 2023 → 30 Jun 2023

Conference

Conference	2023 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference
Abbreviated title	CLEO/Europe-EQEC 2023
Country/Territory	Germany
City	Munich
Period	26/06/23 → 30/06/23

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10.1109/CLEO/EUROPE-EQEC57999.2023.10232362

Cite this

Morawiec, S., Stremplewski, P., Ajduk, A., Kennedy, B., & Szkulmowski, M. (2023). Imaging Intracellular Metabolic Activity of Mammalian Oocytes by Dynamic Full-field Optical Coherence Microscopy. In 2023 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference, CLEO/Europe-EQEC 2023 IEEE, Institute of Electrical and Electronics Engineers. https://doi.org/10.1109/CLEO/EUROPE-EQEC57999.2023.10232362

Morawiec, Seweryn ; Stremplewski, Patrycjusz ; Ajduk, Anna et al. / Imaging Intracellular Metabolic Activity of Mammalian Oocytes by Dynamic Full-field Optical Coherence Microscopy. 2023 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference, CLEO/Europe-EQEC 2023. USA : IEEE, Institute of Electrical and Electronics Engineers, 2023.

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abstract = "Full-field optical coherence microscopy (FF-OCM) is ideally suited to study living cells as it provides high-resolution cross-section images with no need for fluorescent labelling [1,2]. The OCM signal is proportional both to the scattering potential of the sample, providing structural information, and to the motion amplitude of the inter-cellular structures. Thus, the metabolic activity information can be extracted from the spatiotemporal analysis of the OCM images. This technique is particularly appealing for embryology to noninvasively assess the development potential of living cells, e.g., oocytes [3].",

author = "Seweryn Morawiec and Patrycjusz Stremplewski and Anna Ajduk and Brendan Kennedy and Maciej Szkulmowski",

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Morawiec, S, Stremplewski, P, Ajduk, A, Kennedy, B & Szkulmowski, M 2023, Imaging Intracellular Metabolic Activity of Mammalian Oocytes by Dynamic Full-field Optical Coherence Microscopy. in 2023 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference, CLEO/Europe-EQEC 2023. IEEE, Institute of Electrical and Electronics Engineers, USA, 2023 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference, Munich, Germany, 26/06/23. https://doi.org/10.1109/CLEO/EUROPE-EQEC57999.2023.10232362

Imaging Intracellular Metabolic Activity of Mammalian Oocytes by Dynamic Full-field Optical Coherence Microscopy. / Morawiec, Seweryn; Stremplewski, Patrycjusz; Ajduk, Anna et al.
2023 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference, CLEO/Europe-EQEC 2023. USA: IEEE, Institute of Electrical and Electronics Engineers, 2023.

Research output: Chapter in Book/Conference paper › Conference paper › peer-review

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AB - Full-field optical coherence microscopy (FF-OCM) is ideally suited to study living cells as it provides high-resolution cross-section images with no need for fluorescent labelling [1,2]. The OCM signal is proportional both to the scattering potential of the sample, providing structural information, and to the motion amplitude of the inter-cellular structures. Thus, the metabolic activity information can be extracted from the spatiotemporal analysis of the OCM images. This technique is particularly appealing for embryology to noninvasively assess the development potential of living cells, e.g., oocytes [3].

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Morawiec S, Stremplewski P, Ajduk A, Kennedy B, Szkulmowski M. Imaging Intracellular Metabolic Activity of Mammalian Oocytes by Dynamic Full-field Optical Coherence Microscopy. In 2023 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference, CLEO/Europe-EQEC 2023. USA: IEEE, Institute of Electrical and Electronics Engineers. 2023 doi: 10.1109/CLEO/EUROPE-EQEC57999.2023.10232362

Imaging Intracellular Metabolic Activity of Mammalian Oocytes by Dynamic Full-field Optical Coherence Microscopy

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