TY - JOUR
T1 - Image analysis for the quantitative comparison of stress fibers and focal adhesions
AU - Elosegui-Artola, Alberto
AU - Jorge-Peñas, Alvaro
AU - Moreno-Arotzena, Oihana
AU - Oregi, Amaia
AU - Lasa, Marta
AU - Garcéa-Aznar, José Manuel
AU - De Juan-Pardo, Elena M.
AU - Aldabe, Rafael
PY - 2014/9/30
Y1 - 2014/9/30
N2 - Actin stress fibers (SFs) detect and transmit forces to the extracellular matrix through focal adhesions (FAs), and molecules in this pathway determine cellular behavior. Here, we designed two different computational tools to quantify actin SFs and the distribution of actin cytoskeletal proteins within a normalized cellular morphology. Moreover, a systematic cell response comparison between the control cells and those with impaired actin cytoskeleton polymerization was performed to demonstrate the reliability of the tools. Indeed, a variety of proteins that were present within the string beginning at the focal adhesions (vinculin) up to the actin SFs contraction (non-muscle myosin II (NMMII)) were analyzed. Finally, the software used allows for the quantification of the SFs based on the relative positions of FAs. Therefore, it provides a better insight into the cell mechanics and broadens the knowledge of the nature of SFs.
AB - Actin stress fibers (SFs) detect and transmit forces to the extracellular matrix through focal adhesions (FAs), and molecules in this pathway determine cellular behavior. Here, we designed two different computational tools to quantify actin SFs and the distribution of actin cytoskeletal proteins within a normalized cellular morphology. Moreover, a systematic cell response comparison between the control cells and those with impaired actin cytoskeleton polymerization was performed to demonstrate the reliability of the tools. Indeed, a variety of proteins that were present within the string beginning at the focal adhesions (vinculin) up to the actin SFs contraction (non-muscle myosin II (NMMII)) were analyzed. Finally, the software used allows for the quantification of the SFs based on the relative positions of FAs. Therefore, it provides a better insight into the cell mechanics and broadens the knowledge of the nature of SFs.
UR - http://www.scopus.com/inward/record.url?scp=84907480649&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0107393
DO - 10.1371/journal.pone.0107393
M3 - Article
C2 - 25269086
AN - SCOPUS:84907480649
SN - 1932-6203
VL - 9
JO - PLoS One
JF - PLoS One
IS - 9
M1 - e107393
ER -