Illuminating insights into protein-protein interactions using bioluminescence resonance energy transfer (BRET)

Kevin Pfleger, Karin Eidne

Research output: Contribution to journalArticlepeer-review

409 Citations (Scopus)

Abstract

Bioluminescence resonance energy transfer (BRET) is a straightforward biophysical technique for studying protein-protein interactions. It requires: (1) that proteins of interest and suitable controls be labeled with either a donor or acceptor molecule, (2) placement of these labeled proteins in the desired environment for assessing their potential interaction, and (3) use of suitable detection instrumentation to monitor resultant energy transfer. There are now several possible applications, combinations of donor and acceptor molecules, potential assay environments and detection system perturbations. Therefore, this review aims to demystify and clarify the important aspects of the BRET methodology that should be considered when using this technique.
Original languageEnglish
Pages (from-to)165-174
JournalNature Methods
Volume3
Issue number3
DOIs
Publication statusPublished - 2006

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