In vivo measurement of protein thiol oxidation in skeletal muscle

Amber Boyatzis

doi:10.26182/j0qh-6m95

In vivo measurement of protein thiol oxidation in skeletal muscle

Amber Boyatzis

Research output: Thesis › Doctoral Thesis

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Abstract

The aim of this work was to establish whether the thiol groups of proteins were a key target of reactive oxygen species as they are contributors to the pathology of many diseases. Through the development, refinement and application of novel biochemical tools, this work demonstrated that protein thiol oxidation was perturbed in people with Chronic Fatigue Syndrome. In canine and murine models of Duchenne Muscular Dystrophy, novel proteins undergoing thiol oxidation were identified. Using the novel tools developed in this work, it will be possible to better understand the mechanism by which reactive oxygen species cause pathology in disease.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	The University of Western Australia
Supervisors/Advisors	Fournier, Paul, Supervisor Arthur, Peter, Supervisor
Award date	3 Apr 2020
DOIs	https://doi.org/10.26182/j0qh-6m95
Publication status	Unpublished - 2020

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Embargoed from 25/06/2020 to 01/07/2022. Made publicly available on 01/07/2022.

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10.26182/j0qh-6m95

THESIS - DOCTOR OF PHILOSOPHY - BOYATZIS Amber Elizabeth - 2020
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Cite this

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keywords = "Protein thiol oxidation, Duchenne Muscular Dystrophy, Oxidative stress, Skeletal Muscle, Mass spectrometry, Myalgic Encephaiomyelitis/Chronic Fatigue Syndrome",

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AB - The aim of this work was to establish whether the thiol groups of proteins were a key target of reactive oxygen species as they are contributors to the pathology of many diseases. Through the development, refinement and application of novel biochemical tools, this work demonstrated that protein thiol oxidation was perturbed in people with Chronic Fatigue Syndrome. In canine and murine models of Duchenne Muscular Dystrophy, novel proteins undergoing thiol oxidation were identified. Using the novel tools developed in this work, it will be possible to better understand the mechanism by which reactive oxygen species cause pathology in disease.

KW - Protein thiol oxidation

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KW - Oxidative stress

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KW - Mass spectrometry

KW - Myalgic Encephaiomyelitis/Chronic Fatigue Syndrome

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In vivo measurement of protein thiol oxidation in skeletal muscle

Abstract

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