IgG responses to pneumococcal and haemophilus influenzae protein antigens are not impaired in children with a history of recurrent acute otitis media

Selma Wiertsema, Karli Corscadden, Eva Mowe, Brad Zhang, Shyan Vijayasekaran, Harvey Coates, T.J. Mitchell, Wayne Thomas, Peter Richmond, Lea-Ann Kirkham

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Abstract

Background: Vaccines including conserved antigens from Streptococcus pneumoniae and nontypeable Haemophilusinfluenzae (NTHi) have the potential to reduce the burden of acute otitis media. Little is known about the antibody responseto such antigens in young children with recurrent acute otitis media, however, it has been suggested antibody productionmay be impaired in these children.Methods: We measured serum IgG levels against 4 pneumococcal (PspA1, PspA 2, CbpA and Ply) and 3 NTHi (P4, P6 and PD)proteins in a cross-sectional study of 172 children under 3 years of age with a history of recurrent acute otitis media (median7 episodes, requiring ventilation tube insertion) and 63 healthy age-matched controls, using a newly developed multiplexbead assay.Results: Children with a history of recurrent acute otitis media had significantly higher geometric mean serum IgG levelsagainst NTHi proteins P4, P6 and PD compared with healthy controls, whereas there was no difference in antibody levelsagainst pneumococcal protein antigens. In both children with and without a history of acute otitis media, antibody levelsincreased with age and were significantly higher in children colonised with S. pneumoniae or NTHi compared with children that were not colonised. Conclusions: Proteins from S. pneumoniae and NTHi induce serum IgG in children with a history of acute otitis media. The mechanisms in which proteins induce immunity and potential protection requires further investigation but the dogma of impaired antibody responses in children with recurrent acute otitis media should be reconsidered.
Original languageEnglish
Pages (from-to)e49061
JournalPLoS One
Volume7
Issue number11
DOIs
Publication statusPublished - 12 Dec 2012

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otitis media
Haemophilus influenzae
Otitis Media
Immunoglobulin G
antigens
Antigens
Antibodies
Streptococcus pneumoniae
Proteins
proteins
antibodies
Protein S
Ventilation
Assays
Serum
Vaccines
cross-sectional studies
Antibody Formation
Immunity
Pneumonia

Cite this

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title = "IgG responses to pneumococcal and haemophilus influenzae protein antigens are not impaired in children with a history of recurrent acute otitis media",
abstract = "Background: Vaccines including conserved antigens from Streptococcus pneumoniae and nontypeable Haemophilusinfluenzae (NTHi) have the potential to reduce the burden of acute otitis media. Little is known about the antibody responseto such antigens in young children with recurrent acute otitis media, however, it has been suggested antibody productionmay be impaired in these children.Methods: We measured serum IgG levels against 4 pneumococcal (PspA1, PspA 2, CbpA and Ply) and 3 NTHi (P4, P6 and PD)proteins in a cross-sectional study of 172 children under 3 years of age with a history of recurrent acute otitis media (median7 episodes, requiring ventilation tube insertion) and 63 healthy age-matched controls, using a newly developed multiplexbead assay.Results: Children with a history of recurrent acute otitis media had significantly higher geometric mean serum IgG levelsagainst NTHi proteins P4, P6 and PD compared with healthy controls, whereas there was no difference in antibody levelsagainst pneumococcal protein antigens. In both children with and without a history of acute otitis media, antibody levelsincreased with age and were significantly higher in children colonised with S. pneumoniae or NTHi compared with children that were not colonised. Conclusions: Proteins from S. pneumoniae and NTHi induce serum IgG in children with a history of acute otitis media. The mechanisms in which proteins induce immunity and potential protection requires further investigation but the dogma of impaired antibody responses in children with recurrent acute otitis media should be reconsidered.",
author = "Selma Wiertsema and Karli Corscadden and Eva Mowe and Brad Zhang and Shyan Vijayasekaran and Harvey Coates and T.J. Mitchell and Wayne Thomas and Peter Richmond and Lea-Ann Kirkham",
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month = "12",
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TY - JOUR

T1 - IgG responses to pneumococcal and haemophilus influenzae protein antigens are not impaired in children with a history of recurrent acute otitis media

AU - Wiertsema, Selma

AU - Corscadden, Karli

AU - Mowe, Eva

AU - Zhang, Brad

AU - Vijayasekaran, Shyan

AU - Coates, Harvey

AU - Mitchell, T.J.

AU - Thomas, Wayne

AU - Richmond, Peter

AU - Kirkham, Lea-Ann

PY - 2012/12/12

Y1 - 2012/12/12

N2 - Background: Vaccines including conserved antigens from Streptococcus pneumoniae and nontypeable Haemophilusinfluenzae (NTHi) have the potential to reduce the burden of acute otitis media. Little is known about the antibody responseto such antigens in young children with recurrent acute otitis media, however, it has been suggested antibody productionmay be impaired in these children.Methods: We measured serum IgG levels against 4 pneumococcal (PspA1, PspA 2, CbpA and Ply) and 3 NTHi (P4, P6 and PD)proteins in a cross-sectional study of 172 children under 3 years of age with a history of recurrent acute otitis media (median7 episodes, requiring ventilation tube insertion) and 63 healthy age-matched controls, using a newly developed multiplexbead assay.Results: Children with a history of recurrent acute otitis media had significantly higher geometric mean serum IgG levelsagainst NTHi proteins P4, P6 and PD compared with healthy controls, whereas there was no difference in antibody levelsagainst pneumococcal protein antigens. In both children with and without a history of acute otitis media, antibody levelsincreased with age and were significantly higher in children colonised with S. pneumoniae or NTHi compared with children that were not colonised. Conclusions: Proteins from S. pneumoniae and NTHi induce serum IgG in children with a history of acute otitis media. The mechanisms in which proteins induce immunity and potential protection requires further investigation but the dogma of impaired antibody responses in children with recurrent acute otitis media should be reconsidered.

AB - Background: Vaccines including conserved antigens from Streptococcus pneumoniae and nontypeable Haemophilusinfluenzae (NTHi) have the potential to reduce the burden of acute otitis media. Little is known about the antibody responseto such antigens in young children with recurrent acute otitis media, however, it has been suggested antibody productionmay be impaired in these children.Methods: We measured serum IgG levels against 4 pneumococcal (PspA1, PspA 2, CbpA and Ply) and 3 NTHi (P4, P6 and PD)proteins in a cross-sectional study of 172 children under 3 years of age with a history of recurrent acute otitis media (median7 episodes, requiring ventilation tube insertion) and 63 healthy age-matched controls, using a newly developed multiplexbead assay.Results: Children with a history of recurrent acute otitis media had significantly higher geometric mean serum IgG levelsagainst NTHi proteins P4, P6 and PD compared with healthy controls, whereas there was no difference in antibody levelsagainst pneumococcal protein antigens. In both children with and without a history of acute otitis media, antibody levelsincreased with age and were significantly higher in children colonised with S. pneumoniae or NTHi compared with children that were not colonised. Conclusions: Proteins from S. pneumoniae and NTHi induce serum IgG in children with a history of acute otitis media. The mechanisms in which proteins induce immunity and potential protection requires further investigation but the dogma of impaired antibody responses in children with recurrent acute otitis media should be reconsidered.

U2 - 10.1371/journal.pone.0049061

DO - 10.1371/journal.pone.0049061

M3 - Article

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JO - P L o S One

JF - P L o S One

SN - 1932-6203

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ER -