Identification of the fertility restoration locus, Rfo, in radish, as a member of the pentatricopeptide-repeat protein family

S. Desloire; H. Gherbi; W. Laloui; S. Marhadour; V. Clouet; L. Cattolico; C. Falentin; S. Giancola; M. Renard; F. Budar; Ian Small; M. Caboche; R. Delourme; A. Bendahmane

doi:10.1038/sj.embor.embor848

Identification of the fertility restoration locus, Rfo, in radish, as a member of the pentatricopeptide-repeat protein family

S. Desloire, H. Gherbi, W. Laloui, S. Marhadour, V. Clouet, L. Cattolico, C. Falentin, S. Giancola, M. Renard, F. Budar, Ian Small, M. Caboche, R. Delourme, A. Bendahmane

ARC Centre for Plant Energy Biology

Research output: Contribution to journal › Article › peer-review

278 Citations (Scopus)

Abstract

Ogura cytoplasmic male sterility (CMS) in radish (Raphanussativus) is caused by an aberrant mitochondrial gene, Orf138, thatprevents the production of functional pollen without affectingfemale fertility. Rfo, a nuclear gene that restores male fertility,alters the expression of Orf138 at the post-transcriptional level. TheOgura CMS/Rfo two-component system is a useful model for investigatingnuclear–cytoplasmic interactions, as well as the physiologicalbasis of fertility restoration. Using a combination of positionalcloning and microsynteny analysis of Arabidopsis thaliana andradish, we genetically and physically delimited the Rfo locus to a15-kb DNA segment. Analysis of this segment shows that Rfo isa member of the pentatricopeptide repeat (PPR) family. InArabidopsis, this family contains more than 450 members ofunknown function, although most of them are predicted to be targetedto mitochondria and chloroplasts and are thought to haveroles in organellar gene expression.

Original language	English
Pages (from-to)	588-594
Journal	EMBO Reports
Volume	4
Issue number	6
DOIs	https://doi.org/10.1038/sj.embor.embor848
Publication status	Published - 2003

Access to Document

10.1038/sj.embor.embor848

Cite this

Desloire, S., Gherbi, H., Laloui, W., Marhadour, S., Clouet, V., Cattolico, L., Falentin, C., Giancola, S., Renard, M., Budar, F., Small, I., Caboche, M., Delourme, R., & Bendahmane, A. (2003). Identification of the fertility restoration locus, Rfo, in radish, as a member of the pentatricopeptide-repeat protein family. EMBO Reports, 4(6), 588-594. https://doi.org/10.1038/sj.embor.embor848

@article{29a2381d68074389be047ed34c12d5bd,

title = "Identification of the fertility restoration locus, Rfo, in radish, as a member of the pentatricopeptide-repeat protein family",

abstract = "Ogura cytoplasmic male sterility (CMS) in radish (Raphanussativus) is caused by an aberrant mitochondrial gene, Orf138, thatprevents the production of functional pollen without affectingfemale fertility. Rfo, a nuclear gene that restores male fertility,alters the expression of Orf138 at the post-transcriptional level. TheOgura CMS/Rfo two-component system is a useful model for investigatingnuclear–cytoplasmic interactions, as well as the physiologicalbasis of fertility restoration. Using a combination of positionalcloning and microsynteny analysis of Arabidopsis thaliana andradish, we genetically and physically delimited the Rfo locus to a15-kb DNA segment. Analysis of this segment shows that Rfo isa member of the pentatricopeptide repeat (PPR) family. InArabidopsis, this family contains more than 450 members ofunknown function, although most of them are predicted to be targetedto mitochondria and chloroplasts and are thought to haveroles in organellar gene expression.",

author = "S. Desloire and H. Gherbi and W. Laloui and S. Marhadour and V. Clouet and L. Cattolico and C. Falentin and S. Giancola and M. Renard and F. Budar and Ian Small and M. Caboche and R. Delourme and A. Bendahmane",

year = "2003",

doi = "10.1038/sj.embor.embor848",

language = "English",

volume = "4",

pages = "588--594",

journal = "EMBO Reports",

issn = "1469-221X",

publisher = "Nature Publishing Group",

number = "6",

}

Desloire, S, Gherbi, H, Laloui, W, Marhadour, S, Clouet, V, Cattolico, L, Falentin, C, Giancola, S, Renard, M, Budar, F, Small, I, Caboche, M, Delourme, R & Bendahmane, A 2003, 'Identification of the fertility restoration locus, Rfo, in radish, as a member of the pentatricopeptide-repeat protein family', EMBO Reports, vol. 4, no. 6, pp. 588-594. https://doi.org/10.1038/sj.embor.embor848

TY - JOUR

T1 - Identification of the fertility restoration locus, Rfo, in radish, as a member of the pentatricopeptide-repeat protein family

AU - Desloire, S.

AU - Gherbi, H.

AU - Laloui, W.

AU - Marhadour, S.

AU - Clouet, V.

AU - Cattolico, L.

AU - Falentin, C.

AU - Giancola, S.

AU - Renard, M.

AU - Budar, F.

AU - Small, Ian

AU - Caboche, M.

AU - Delourme, R.

AU - Bendahmane, A.

PY - 2003

Y1 - 2003

N2 - Ogura cytoplasmic male sterility (CMS) in radish (Raphanussativus) is caused by an aberrant mitochondrial gene, Orf138, thatprevents the production of functional pollen without affectingfemale fertility. Rfo, a nuclear gene that restores male fertility,alters the expression of Orf138 at the post-transcriptional level. TheOgura CMS/Rfo two-component system is a useful model for investigatingnuclear–cytoplasmic interactions, as well as the physiologicalbasis of fertility restoration. Using a combination of positionalcloning and microsynteny analysis of Arabidopsis thaliana andradish, we genetically and physically delimited the Rfo locus to a15-kb DNA segment. Analysis of this segment shows that Rfo isa member of the pentatricopeptide repeat (PPR) family. InArabidopsis, this family contains more than 450 members ofunknown function, although most of them are predicted to be targetedto mitochondria and chloroplasts and are thought to haveroles in organellar gene expression.

AB - Ogura cytoplasmic male sterility (CMS) in radish (Raphanussativus) is caused by an aberrant mitochondrial gene, Orf138, thatprevents the production of functional pollen without affectingfemale fertility. Rfo, a nuclear gene that restores male fertility,alters the expression of Orf138 at the post-transcriptional level. TheOgura CMS/Rfo two-component system is a useful model for investigatingnuclear–cytoplasmic interactions, as well as the physiologicalbasis of fertility restoration. Using a combination of positionalcloning and microsynteny analysis of Arabidopsis thaliana andradish, we genetically and physically delimited the Rfo locus to a15-kb DNA segment. Analysis of this segment shows that Rfo isa member of the pentatricopeptide repeat (PPR) family. InArabidopsis, this family contains more than 450 members ofunknown function, although most of them are predicted to be targetedto mitochondria and chloroplasts and are thought to haveroles in organellar gene expression.

U2 - 10.1038/sj.embor.embor848

DO - 10.1038/sj.embor.embor848

M3 - Article

SN - 1469-221X

VL - 4

SP - 588

EP - 594

JO - EMBO Reports

JF - EMBO Reports

IS - 6

ER -

Identification of the fertility restoration locus, Rfo, in radish, as a member of the pentatricopeptide-repeat protein family

Abstract

Access to Document

Fingerprint

Cite this