Identification of Qualitative Resistance Genes in Brassica napus against Blackleg

Yueqi Zhang

doi:10.26182/qwbj-mz53

Identification of Qualitative Resistance Genes in Brassica napus against Blackleg

Yueqi Zhang

School of Biological Sciences

Research output: Thesis › Doctoral Thesis

120 Downloads (Pure)

Abstract

Canola (Brassica napus) production is threatened by blackleg disease worldwide. Identification of qualitative resistance genes is crucial for facilitating durable disease management. In my PhD project, strong candidates for Rlm3, Rlm4 and Rlm7 were identified. Gene relatives of Rlm3, Rlm4, Rlm7 and Rlm9 were analysed to gain insights on their evolution and functions. Reliable and high-throughput genetic screening markers for Rlm3, Rlm4, Rlm7 and Rlm9 were developed to assist canola breeding. A protocol for flow sorting single chromosomes for canola was established for the first time which laid the foundation for chromosome genomics in Brassica.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	The University of Western Australia
Supervisors/Advisors	Batley, Jacqueline, Supervisor Edwards, Dave, Supervisor Doležel, Jaroslav, Supervisor, External person
Award date	10 Mar 2021
DOIs	https://doi.org/10.26182/qwbj-mz53
Publication status	Unpublished - 2021

Embargo information

Embargoed from 23/03/2021 to 10/03/2023. Made publicly available on 10/03/2023.

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10.26182/qwbj-mz53

THESIS - DOCTOR OF PHILOSOPHY - ZHANG, Yueqi - 2021
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2 Review article
1 Chapter

Comparison and evolutionary analysis of Brassica nucleotide binding site leucine rich repeat (NLR) genes and importance for disease resistance breeding
Zhang, Y., Edwards, D. & Batley, J., Mar 2021, In: Plant Genome. 14, 1, 12 p., e20060.
Research output: Contribution to journal › Review article › peer-review

Open Access
5 Citations (Scopus)
Frontiers in dissecting and managing brassica diseases: From reference‐based rga candidate identification to building pan‐rgaomes
Zhang, Y., Thomas, W., Bayer, P. E., Edwards, D. & Batley, J., 1 Dec 2020, In: International Journal of Molecular Sciences. 21, 23, p. 1-14 14 p., 8964.
Research output: Contribution to journal › Review article › peer-review

Open Access
14 Citations (Scopus)
Genome-wide mining of disease resistance gene analogs using conserved domains
Tirnaz, S., Zhang, Y. & Batley, J., 2020, Methods in Molecular Biology. 1 ed. USA: Humana Press Inc., p. 365-375 11 p. (Methods in Molecular Biology; vol. 2107).
Research output: Chapter in Book/Conference paper › Chapter › peer-review
4 Citations (Scopus)

Cite this

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title = "Identification of Qualitative Resistance Genes in Brassica napus against Blackleg",

abstract = "Canola (Brassica napus) production is threatened by blackleg disease worldwide. Identification of qualitative resistance genes is crucial for facilitating durable disease management. In my PhD project, strong candidates for Rlm3, Rlm4 and Rlm7 were identified. Gene relatives of Rlm3, Rlm4, Rlm7 and Rlm9 were analysed to gain insights on their evolution and functions. Reliable and high-throughput genetic screening markers for Rlm3, Rlm4, Rlm7 and Rlm9 were developed to assist canola breeding. A protocol for flow sorting single chromosomes for canola was established for the first time which laid the foundation for chromosome genomics in Brassica.",

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school = "The University of Western Australia",

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AB - Canola (Brassica napus) production is threatened by blackleg disease worldwide. Identification of qualitative resistance genes is crucial for facilitating durable disease management. In my PhD project, strong candidates for Rlm3, Rlm4 and Rlm7 were identified. Gene relatives of Rlm3, Rlm4, Rlm7 and Rlm9 were analysed to gain insights on their evolution and functions. Reliable and high-throughput genetic screening markers for Rlm3, Rlm4, Rlm7 and Rlm9 were developed to assist canola breeding. A protocol for flow sorting single chromosomes for canola was established for the first time which laid the foundation for chromosome genomics in Brassica.

KW - canola

KW - blackleg qualitative resistance genes

KW - resequencing

KW - genetic screening markers

KW - chromosome

KW - flow cytometry

U2 - 10.26182/qwbj-mz53

DO - 10.26182/qwbj-mz53

M3 - Doctoral Thesis

ER -

Identification of Qualitative Resistance Genes in Brassica napus against Blackleg

Abstract

Embargo information

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Research output

Comparison and evolutionary analysis of Brassica nucleotide binding site leucine rich repeat (NLR) genes and importance for disease resistance breeding

Frontiers in dissecting and managing brassica diseases: From reference‐based rga candidate identification to building pan‐rgaomes

Genome-wide mining of disease resistance gene analogs using conserved domains

Cite this