Identification of new metribuzin-tolerant wheat (Triticum spp.) genotypes

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Herbicide-tolerant wheats are preferred for effective weed management. Rapid phenotyping and effective differential dose are vital for the identification of tolerant genotypes among large quantities of genetic resources. A sand-tray system has been developed to enable rapid assessment of metribuzin damage in wheat seedlings. In total, 946 wheat genotypes were evaluated for metribuzin tolerance by using this system under control and metribuzin-treated conditions. SPAD chlorophyll content index (CCI) offered a non-destructive and rapid analysis of leaf chlorophyll content in wheat seedlings. The application rate for 50% reduction in SPAD CCI (I50) was 3.2-fold higher in the current tolerant genotype (Eagle Rock) than the susceptible genotype Spear. A confirmed dose of 800g a.i. ha-1 could differentiate between metribuzin-tolerant and -susceptible lines. The experimental design with two-directional blocking followed by statistical analysis to model the spatial variation was instrumental in selecting potential tolerant or susceptible genotypes. Metribuzin reduced chlorophyll by 51.4% in treated seedlings. The overall adjusted mean SPAD CCI ranged from 13.5 to 42.7 for control (untreated) plants and from 0.1 to 29.9 for treated plants. Through repeated validation, nine genotypes had higher chlorophyll content after metribuzin treatment and significantly (P

Original languageEnglish
Pages (from-to)401-408
Number of pages8
JournalCrop and Pasture Science
Volume68
Issue number5
DOIs
Publication statusPublished - 19 May 2017

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metribuzin
Triticum
chlorophyll
wheat
genotype
seedlings
eagles
dosage
trays
genetic resources
application rate
spatial variation
weed control
statistical analysis
herbicides
experimental design
rocks
sand
phenotype
leaves

Cite this

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title = "Identification of new metribuzin-tolerant wheat (Triticum spp.) genotypes",
abstract = "Herbicide-tolerant wheats are preferred for effective weed management. Rapid phenotyping and effective differential dose are vital for the identification of tolerant genotypes among large quantities of genetic resources. A sand-tray system has been developed to enable rapid assessment of metribuzin damage in wheat seedlings. In total, 946 wheat genotypes were evaluated for metribuzin tolerance by using this system under control and metribuzin-treated conditions. SPAD chlorophyll content index (CCI) offered a non-destructive and rapid analysis of leaf chlorophyll content in wheat seedlings. The application rate for 50{\%} reduction in SPAD CCI (I50) was 3.2-fold higher in the current tolerant genotype (Eagle Rock) than the susceptible genotype Spear. A confirmed dose of 800g a.i. ha-1 could differentiate between metribuzin-tolerant and -susceptible lines. The experimental design with two-directional blocking followed by statistical analysis to model the spatial variation was instrumental in selecting potential tolerant or susceptible genotypes. Metribuzin reduced chlorophyll by 51.4{\%} in treated seedlings. The overall adjusted mean SPAD CCI ranged from 13.5 to 42.7 for control (untreated) plants and from 0.1 to 29.9 for treated plants. Through repeated validation, nine genotypes had higher chlorophyll content after metribuzin treatment and significantly (P",
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Identification of new metribuzin-tolerant wheat (Triticum spp.) genotypes. / Bhoite, Roopali N.; Si, Ping; Stefanova, Katia T.; Siddique, Kadambot H.M.; Yan, Guijun.

In: Crop and Pasture Science, Vol. 68, No. 5, 19.05.2017, p. 401-408.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Identification of new metribuzin-tolerant wheat (Triticum spp.) genotypes

AU - Bhoite, Roopali N.

AU - Si, Ping

AU - Stefanova, Katia T.

AU - Siddique, Kadambot H.M.

AU - Yan, Guijun

PY - 2017/5/19

Y1 - 2017/5/19

N2 - Herbicide-tolerant wheats are preferred for effective weed management. Rapid phenotyping and effective differential dose are vital for the identification of tolerant genotypes among large quantities of genetic resources. A sand-tray system has been developed to enable rapid assessment of metribuzin damage in wheat seedlings. In total, 946 wheat genotypes were evaluated for metribuzin tolerance by using this system under control and metribuzin-treated conditions. SPAD chlorophyll content index (CCI) offered a non-destructive and rapid analysis of leaf chlorophyll content in wheat seedlings. The application rate for 50% reduction in SPAD CCI (I50) was 3.2-fold higher in the current tolerant genotype (Eagle Rock) than the susceptible genotype Spear. A confirmed dose of 800g a.i. ha-1 could differentiate between metribuzin-tolerant and -susceptible lines. The experimental design with two-directional blocking followed by statistical analysis to model the spatial variation was instrumental in selecting potential tolerant or susceptible genotypes. Metribuzin reduced chlorophyll by 51.4% in treated seedlings. The overall adjusted mean SPAD CCI ranged from 13.5 to 42.7 for control (untreated) plants and from 0.1 to 29.9 for treated plants. Through repeated validation, nine genotypes had higher chlorophyll content after metribuzin treatment and significantly (P

AB - Herbicide-tolerant wheats are preferred for effective weed management. Rapid phenotyping and effective differential dose are vital for the identification of tolerant genotypes among large quantities of genetic resources. A sand-tray system has been developed to enable rapid assessment of metribuzin damage in wheat seedlings. In total, 946 wheat genotypes were evaluated for metribuzin tolerance by using this system under control and metribuzin-treated conditions. SPAD chlorophyll content index (CCI) offered a non-destructive and rapid analysis of leaf chlorophyll content in wheat seedlings. The application rate for 50% reduction in SPAD CCI (I50) was 3.2-fold higher in the current tolerant genotype (Eagle Rock) than the susceptible genotype Spear. A confirmed dose of 800g a.i. ha-1 could differentiate between metribuzin-tolerant and -susceptible lines. The experimental design with two-directional blocking followed by statistical analysis to model the spatial variation was instrumental in selecting potential tolerant or susceptible genotypes. Metribuzin reduced chlorophyll by 51.4% in treated seedlings. The overall adjusted mean SPAD CCI ranged from 13.5 to 42.7 for control (untreated) plants and from 0.1 to 29.9 for treated plants. Through repeated validation, nine genotypes had higher chlorophyll content after metribuzin treatment and significantly (P

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