TY - JOUR
T1 - Identification of candidate genes for LepR1 resistance against Leptosphaeria maculans in Brassica napus
AU - Cantila, Aldrin Y.
AU - Thomas, William J.W.
AU - Saad, Nur Shuhadah Mohd
AU - Severn-Ellis, Anita A.
AU - Anderson, Robyn
AU - Bayer, Philipp E.
AU - Edwards, David
AU - Van de Wouw, Angela P.
AU - Batley, Jacqueline
N1 - Funding Information:
This study is funded by the Australian Research Council projects DP200100762, and DP210100296 and the Grains Research and Development Corporation (UWA1905-006RTX). Acknowledgments
Publisher Copyright:
Copyright © 2023 Cantila, Thomas, Saad, Severn-Ellis, Anderson, Bayer, Edwards, Van de Wouw and Batley.
PY - 2023/2/14
Y1 - 2023/2/14
N2 - Utilising resistance (R) genes, such as LepR1, against Leptosphaeria maculans, the causal agent of blackleg in canola (Brassica napus), could help manage the disease in the field and increase crop yield. Here we present a genome wide association study (GWAS) in B. napus to identify LepR1 candidate genes. Disease phenotyping of 104 B. napus genotypes revealed 30 resistant and 74 susceptible lines. Whole genome re-sequencing of these cultivars yielded over 3 million high quality single nucleotide polymorphisms (SNPs). GWAS in mixed linear model (MLM) revealed a total of 2,166 significant SNPs associated with LepR1 resistance. Of these SNPs, 2108 (97%) were found on chromosome A02 of B. napus cv. Darmor bzh v9 with a delineated LepR1_mlm1 QTL at 15.11-26.08 Mb. In LepR1_mlm1, there are 30 resistance gene analogs (RGAs) (13 nucleotide-binding site-leucine rich repeats (NLRs), 12 receptor-like kinases (RLKs), and 5 transmembrane-coiled-coil (TM-CCs)). Sequence analysis of alleles in resistant and susceptible lines was undertaken to identify candidate genes. This research provides insights into blackleg resistance in B. napus and assists identification of the functional LepR1 blackleg resistance gene.
AB - Utilising resistance (R) genes, such as LepR1, against Leptosphaeria maculans, the causal agent of blackleg in canola (Brassica napus), could help manage the disease in the field and increase crop yield. Here we present a genome wide association study (GWAS) in B. napus to identify LepR1 candidate genes. Disease phenotyping of 104 B. napus genotypes revealed 30 resistant and 74 susceptible lines. Whole genome re-sequencing of these cultivars yielded over 3 million high quality single nucleotide polymorphisms (SNPs). GWAS in mixed linear model (MLM) revealed a total of 2,166 significant SNPs associated with LepR1 resistance. Of these SNPs, 2108 (97%) were found on chromosome A02 of B. napus cv. Darmor bzh v9 with a delineated LepR1_mlm1 QTL at 15.11-26.08 Mb. In LepR1_mlm1, there are 30 resistance gene analogs (RGAs) (13 nucleotide-binding site-leucine rich repeats (NLRs), 12 receptor-like kinases (RLKs), and 5 transmembrane-coiled-coil (TM-CCs)). Sequence analysis of alleles in resistant and susceptible lines was undertaken to identify candidate genes. This research provides insights into blackleg resistance in B. napus and assists identification of the functional LepR1 blackleg resistance gene.
KW - association mapping
KW - blackleg resistance
KW - canola
KW - gene sequencing
KW - LepR1 resistant-specific marker
UR - http://www.scopus.com/inward/record.url?scp=85149413153&partnerID=8YFLogxK
U2 - 10.3389/fpls.2023.1051994
DO - 10.3389/fpls.2023.1051994
M3 - Article
C2 - 36866377
AN - SCOPUS:85149413153
VL - 14
JO - Frontiers in Plant Science
JF - Frontiers in Plant Science
SN - 1664-462X
M1 - 1051994
ER -