Identification of an AP-2 Element in the -323 to -285 Region of the TNF-a Gene

K.M. Kroeger, Lawrence Abraham

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

We have identified a region of the human TNF-alpha promoter between nucleotides -323 and -285, capable of influencing transcriptional activity. This region encompasses the -308 polymorphism and contains a 10 bp sequence homologous to the consensus binding site of activator protein-2 (AP-2). Protein complexes derived from U937 and Jurkat cells were found to bind to this element. Competitive EMSA using a consensus AP-2 oligonucleotide indicated that AP-2 may be involved. Functional assays demonstrate that this region can repress activity of a heterologous promoter in the Jurkat T-cell line, but act as an inducible enhancer of transcription in U937 cells.
Original languageEnglish
Pages (from-to)43-51
JournalBiochemistry and Molecular Biology International
Volume40
Issue number1
Publication statusPublished - 1996

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