@article{63dd9812211342f584c24246b505b4da,
title = "Identification of a Major QTL-Controlling Resistance to the Subtropical Race 4 of Fusarium oxysporum f. sp. cubense in Musa acuminata ssp. malaccensis",
abstract = "Vascular wilt caused by the ascomycete fungal pathogen Fusarium oxysporum f. sp. cubense (Foc) is a major constraint of banana production around the world. The virulent race, namely Tropical Race 4, can infect all Cavendish-type banana plants and is now widespread across the globe, causing devastating losses to global banana production. In this study, we characterized Foc Subtropical Race 4 (STR4) resistance in a wild banana relative which, through estimated genome size and ancestry analysis, was confirmed to be Musa acuminata ssp. malaccensis. Using a self-derived F2 population segregating for STR4 resistance, quantitative trait loci sequencing (QTL-seq) was performed on bulks consisting of resistant and susceptible individuals. Changes in SNP index between the bulks revealed a major QTL located on the distal end of the long arm of chromosome 3. Multiple resistance genes are present in this region. Identification of chromosome regions conferring resistance to Foc can facilitate marker assisted selection in breeding programs and paves the way towards identifying genes underpinning resistance.",
keywords = "banana, bulk segregant analysis, Fusarium oxysporum f. sp. cubense, fusarium wilt, host resistance, QTL-seq, quantitative trait locus, Subtropical Race 4",
author = "Andrew Chen and Jiaman Sun and Guillaume Martin and Gray, {Lesley Ann} and Eva H{\v r}ibov{\'a} and Pavla Christelov{\'a} and Nabila Yahiaoui and Steve Rounsley and Rebecca Lyons and Jacqueline Batley and Ning Chen and Sharon Hamill and Rai, {Subash K.} and Lachlan Coin and Brigitte Uwimana and Angelique D{\textquoteright}Hont and Jaroslav Dole{\v z}el and David Edwards and Rony Swennen and Aitken, {Elizabeth A.B.}",
note = "Funding Information: The authors declare no conflict of interest. The authors declare that this study received funding from Bill and Melinda Gates Foundation (OPP1213871). The funder was not involved in the study design, collection, analysis, interpretation of data, the writing of this article or the decision to submit it for publication. Funding Information: This research was funded by Bill and Melinda Gates Foundation, grant number (OPP1213871) and the APC was funded by (OPP1213871). This work was supported, in part, by the Bill and Melinda Gates Foundation (OPP1213871) through a program with the International Institute of Tropical Agriculture. Under the grant conditions of the foundation, a Creative Commons Attribution 4.0 Generic License has already been assigned to the author-accepted manuscript version that might arise from this submission. Research funding support was also provided in part by Hort Innovation Australia (BA 17006). Jiaman Sun is supported by Guangxi Science and Technology Planning Project (Guike AA21196005) and Guangxi Natural Science Foundation (2021GXNSFAA196014). Publisher Copyright: {\textcopyright} 2023 by the authors.",
year = "2023",
month = feb,
doi = "10.3390/pathogens12020289",
language = "English",
volume = "12",
journal = "Pathogens",
issn = "2076-0817",
publisher = "MDPI AG",
number = "2",
}
