Identification and validation of closely linked PCR markers for improving yellow spot resistance in common wheat

Fei Ren

    Research output: ThesisMaster's Thesis

    293 Downloads (Pure)


    Yellow spot (Pyrenophora tritici-repentis) is a major foliar disease in wheat
    (Triticum aestivum) and it has become more serious in recent years, possibly
    due to changes in climate or cultural practice. A major QTL located on the short
    arm of wheat chromosome 2B, which explains 30–40% of the phenotypic
    variance, has been identified recently. However, the closest marker linked to this
    QTL was a DArT marker that is not easy to use in large-scale selection; the
    closest PCR-based marker available (2.7 cM) was too far away to be reliably
    used for tagging the locus in wheat breeding. For these reasons, studies were
    undertaken to develop more closely–linked and user-friendly markers for this
    major QTL. Forty-one new markers either synthesised from DArT markers or
    identified from the GrainGene database were assessed. From these, a new
    PCR-based marker (Rfsts1), located 0.3 cM away from the major QTL, was
    developed for marker assisted selection in yellow spot breeding. This marker
    linked to the 2BS locus was used to combine with another QTL conferring yellow
    spot resistance on chromosome 5BL and the effects of gene pyramiding in
    increasing resistance to this disease were investigated. The results indicated
    that the combined selection could significantly increase selection efficiency and
    accuracy and resulted in genotypes with significantly higher resistance to yellow
    Original languageEnglish
    • Yan, Guijun, Supervisor
    • Barbetti, Martin, Supervisor
    • Liu, Chunji, Supervisor
    Publication statusUnpublished - Apr 2015


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