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Abstract
Zaragozic acid A (1) is a potent cholesterol lowering, polyketide natural product made by various filamentous fungi. The reconstitution of enzymes responsible for the initial steps of the biosynthetic pathway of 1 is accomplished using an engineered fungal heterologous host. These initial steps feature the priming of a benzoic acid starter unit onto a highly reducing polyketide synthase (HRPKS), followed by oxaloacetate extension and product release to generate a tricarboxylic acid containing product 2. The reconstitution studies demonstrated that only three enzymes, HRPKS, citrate synthase, and hydrolase, are needed in A. nidulans to produce the structurally complex product.
Original language | English |
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Pages (from-to) | 3560-3563 |
Number of pages | 4 |
Journal | Organic Letters |
Volume | 19 |
Issue number | 13 |
DOIs | |
Publication status | Published - 7 Jul 2017 |
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Dive into the research topics of 'Identification and Heterologous Production of a Benzoyl-Primed Tricarboxylic Acid Polyketide Intermediate from the Zaragozic Acid A Biosynthetic Pathway'. Together they form a unique fingerprint.Projects
- 1 Finished
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Uncovering the Hidden Secondary Metabolite Biosynthetic Potential of Fungi
1/01/17 → 15/03/21
Project: Research