Abstract
Zaragozic acid A (1) is a potent cholesterol lowering, polyketide natural product made by various filamentous fungi. The reconstitution of enzymes responsible for the initial steps of the biosynthetic pathway of 1 is accomplished using an engineered fungal heterologous host. These initial steps feature the priming of a benzoic acid starter unit onto a highly reducing polyketide synthase (HRPKS), followed by oxaloacetate extension and product release to generate a tricarboxylic acid containing product 2. The reconstitution studies demonstrated that only three enzymes, HRPKS, citrate synthase, and hydrolase, are needed in A. nidulans to produce the structurally complex product.
| Original language | English |
|---|---|
| Pages (from-to) | 3560-3563 |
| Number of pages | 4 |
| Journal | Organic Letters |
| Volume | 19 |
| Issue number | 13 |
| DOIs | |
| Publication status | Published - 7 Jul 2017 |
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Identification and Heterologous Production of a Benzoyl-Primed Tricarboxylic Acid Polyketide Intermediate from the Zaragozic Acid A Biosynthetic Pathway. / Liu, Nicholas; Hung, Yiu Sun; Gao, Shu Shan; Hang, Leibniz; Zou, Yi; Chooi, Yit Heng; Tang, Yi.
In: Organic Letters, Vol. 19, No. 13, 07.07.2017, p. 3560-3563.Research output: Contribution to journal › Article
TY - JOUR
T1 - Identification and Heterologous Production of a Benzoyl-Primed Tricarboxylic Acid Polyketide Intermediate from the Zaragozic Acid A Biosynthetic Pathway
AU - Liu, Nicholas
AU - Hung, Yiu Sun
AU - Gao, Shu Shan
AU - Hang, Leibniz
AU - Zou, Yi
AU - Chooi, Yit Heng
AU - Tang, Yi
PY - 2017/7/7
Y1 - 2017/7/7
N2 - Zaragozic acid A (1) is a potent cholesterol lowering, polyketide natural product made by various filamentous fungi. The reconstitution of enzymes responsible for the initial steps of the biosynthetic pathway of 1 is accomplished using an engineered fungal heterologous host. These initial steps feature the priming of a benzoic acid starter unit onto a highly reducing polyketide synthase (HRPKS), followed by oxaloacetate extension and product release to generate a tricarboxylic acid containing product 2. The reconstitution studies demonstrated that only three enzymes, HRPKS, citrate synthase, and hydrolase, are needed in A. nidulans to produce the structurally complex product.
AB - Zaragozic acid A (1) is a potent cholesterol lowering, polyketide natural product made by various filamentous fungi. The reconstitution of enzymes responsible for the initial steps of the biosynthetic pathway of 1 is accomplished using an engineered fungal heterologous host. These initial steps feature the priming of a benzoic acid starter unit onto a highly reducing polyketide synthase (HRPKS), followed by oxaloacetate extension and product release to generate a tricarboxylic acid containing product 2. The reconstitution studies demonstrated that only three enzymes, HRPKS, citrate synthase, and hydrolase, are needed in A. nidulans to produce the structurally complex product.
UR - http://www.scopus.com/inward/record.url?scp=85022343985&partnerID=8YFLogxK
U2 - 10.1021/acs.orglett.7b01534
DO - 10.1021/acs.orglett.7b01534
M3 - Article
VL - 19
SP - 3560
EP - 3563
JO - Organic Letters
JF - Organic Letters
SN - 1523-7052
IS - 13
ER -