Identification and functional characterisation of non-canonical DNA methylation readers in the mammalian brain

Sufyaan Mohamed

doi:10.26182/wn8w-qa45

Identification and functional characterisation of non-canonical DNA methylation readers in the mammalian brain

Sufyaan Mohamed

School of Molecular Sciences

Research output: Thesis › Doctoral Thesis

198 Downloads (Pure)

Abstract

DNA methylation is a covalent modification occurring predominantly in the CG dinucleotide context within mammals (mCG), but non-CG contexts (mCH, where H = A, C or T) exist within restricted cell types. In the brain, mCH accumulates to become the dominant form of DNA methylation in adult neurons, coinciding with synaptogenesis. Many proteins binding to mCG have been identified and characterised, but this is lacking for mCA. This PhD constitutes the first study that comprehensively identifies proteins that bind to CG and non-CG DNA methylation in human and mouse brain, providing promising protein candidates for future studies to build upon.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	The University of Western Australia
Supervisors/Advisors	Lister, Ryan, Supervisor Bogdanovic, Ozren, Supervisor Karpievitch, Yuliya, Supervisor Small, Ian, Supervisor Murcha, Monika, Supervisor
Thesis sponsors	Australian Government Research Training Program
Award date	14 May 2021
DOIs	https://doi.org/10.26182/wn8w-qa45
Publication status	Unpublished - 2021

Access to Document

10.26182/wn8w-qa45

THESIS - DOCTOR OF PHILOSOPHY - MOHAMED, Sufyaan - 2021
This work is protected by Copyright. You may print or download ONE copy of this document for the purpose of your own non-commercial research or study. Any other use requires permission from the copyright owner. The Copyright Act requires you to attribute any copyright works you quote or paraphrase.

Cite this

@phdthesis{3692c10ec3d44eb2951eca3233d1c424,

title = "Identification and functional characterisation of non-canonical DNA methylation readers in the mammalian brain",

abstract = "DNA methylation is a covalent modification occurring predominantly in the CG dinucleotide context within mammals (mCG), but non-CG contexts (mCH, where H = A, C or T) exist within restricted cell types. In the brain, mCH accumulates to become the dominant form of DNA methylation in adult neurons, coinciding with synaptogenesis. Many proteins binding to mCG have been identified and characterised, but this is lacking for mCA. This PhD constitutes the first study that comprehensively identifies proteins that bind to CG and non-CG DNA methylation in human and mouse brain, providing promising protein candidates for future studies to build upon. ",

keywords = "DNA methylation, Epigenetics, Proteomics, Mass Spectrometry, Neurodevelopment, MECP2, CH methylation, MBD2",

author = "Sufyaan Mohamed",

year = "2021",

doi = "10.26182/wn8w-qa45",

language = "English",

school = "The University of Western Australia",

}

TY - BOOK

T1 - Identification and functional characterisation of non-canonical DNA methylation readers in the mammalian brain

AU - Mohamed, Sufyaan

PY - 2021

Y1 - 2021

N2 - DNA methylation is a covalent modification occurring predominantly in the CG dinucleotide context within mammals (mCG), but non-CG contexts (mCH, where H = A, C or T) exist within restricted cell types. In the brain, mCH accumulates to become the dominant form of DNA methylation in adult neurons, coinciding with synaptogenesis. Many proteins binding to mCG have been identified and characterised, but this is lacking for mCA. This PhD constitutes the first study that comprehensively identifies proteins that bind to CG and non-CG DNA methylation in human and mouse brain, providing promising protein candidates for future studies to build upon.

AB - DNA methylation is a covalent modification occurring predominantly in the CG dinucleotide context within mammals (mCG), but non-CG contexts (mCH, where H = A, C or T) exist within restricted cell types. In the brain, mCH accumulates to become the dominant form of DNA methylation in adult neurons, coinciding with synaptogenesis. Many proteins binding to mCG have been identified and characterised, but this is lacking for mCA. This PhD constitutes the first study that comprehensively identifies proteins that bind to CG and non-CG DNA methylation in human and mouse brain, providing promising protein candidates for future studies to build upon.

KW - DNA methylation

KW - Epigenetics

KW - Proteomics

KW - Mass Spectrometry

KW - Neurodevelopment

KW - MECP2

KW - CH methylation

KW - MBD2

U2 - 10.26182/wn8w-qa45

DO - 10.26182/wn8w-qa45

M3 - Doctoral Thesis

ER -

Identification and functional characterisation of non-canonical DNA methylation readers in the mammalian brain

Abstract

Access to Document

Fingerprint

Cite this