Clostridioides (Clostridium) difficile in the home environment in Perth, Western Australia

Niraj Shivaperumal

doi:10.26182/5j7f-9e47

Clostridioides (Clostridium) difficile in the home environment in Perth, Western Australia

Niraj Shivaperumal

School of Biomedical Sciences

Research output: Thesis › Doctoral Thesis

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Abstract

This research hypothesised that the domestic environment, pets, and food may contribute to the dissemination of Clostridium difficile (CD), leading to CDI in the community. Convenience samples were collected from 23 home gardens, households, and meat samples across various suburbs of Perth, Western Australia. The study unveils a high prevalence of clinically important toxigenic strains with diverse ribotypes and identifies novel strains exclusively producing toxin A. A clinically significant strain, RT002, was detected in cats. Antimicrobial resistance was noted in numerous isolates from various sources. Several novel strains failed to produce black colonies on ChromID agar, exhibiting unique characteristics.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	The University of Western Australia
Supervisors/Advisors	Riley, Thomas, Supervisor Chang, Barbara, Supervisor
Thesis sponsors	Australian Government Research Training Program
Award date	16 Apr 2024
DOIs	https://doi.org/10.26182/5j7f-9e47
Publication status	Unpublished - 2023

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10.26182/5j7f-9e47

THESIS - DOCTOR OF PHILOSOPHY - SHIVAPERUMAL Nirajmohan - 2023
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3 Article
1 Review article

Complete Genome Sequences of Evolutionary Clade C-III Strains of Clostridioides (Clostridium) difficile Isolated from the Environment in Western Australia
Shivaperumal, N., Hain-Saunders, N. M. R., Chang, B. J. J., Riley, T. V. V. & Knight, D. R. R., May 2023, In: Microbiology Resource Announcements. 12, 5, 2 p., e00239-23.
Research output: Contribution to journal › Article › peer-review

Open Access
1 Citation (Scopus)
The Brief Case: a White-Colony-Producing Clostridioides difficile Ribotype 020 Strain
Imwattana, K., Shivaperumal, N., Leepattarakit, T., Kiratisin, P., Knight, D. R. & Riley, T. V., 20 Jun 2023, In: Journal of Clinical Microbiology. 61, 6, e0089322.
Research output: Contribution to journal › Review article › peer-review

Open Access

2 Citations (Scopus)
Esculin hydrolysis negative and TcdA-only producing strains of Clostridium (Clostridiodes) difficile from the environment in Western Australia
Shivaperumal, N., Knight, D. R., Imwattana, K., Androga, G. O., Chang, B. J. & Riley, T. V., Sept 2022, In: Journal of Applied Microbiology. 133, 3, p. 1183-1196 14 p.
Research output: Contribution to journal › Article › peer-review

Open Access
7 Citations (Scopus)

Cite this

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title = "Clostridioides (Clostridium) difficile in the home environment in Perth, Western Australia",

abstract = "This research hypothesised that the domestic environment, pets, and food may contribute to the dissemination of Clostridium difficile (CD), leading to CDI in the community. Convenience samples were collected from 23 home gardens, households, and meat samples across various suburbs of Perth, Western Australia. The study unveils a high prevalence of clinically important toxigenic strains with diverse ribotypes and identifies novel strains exclusively producing toxin A. A clinically significant strain, RT002, was detected in cats. Antimicrobial resistance was noted in numerous isolates from various sources. Several novel strains failed to produce black colonies on ChromID agar, exhibiting unique characteristics.",

keywords = "Clostridioides (Clostridium) difficile, Molecular Epidemiology, Homes, Novel Variant strains, Phenotypic and Genotypic Characterisation, Food and Environment, OneHealth",

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AB - This research hypothesised that the domestic environment, pets, and food may contribute to the dissemination of Clostridium difficile (CD), leading to CDI in the community. Convenience samples were collected from 23 home gardens, households, and meat samples across various suburbs of Perth, Western Australia. The study unveils a high prevalence of clinically important toxigenic strains with diverse ribotypes and identifies novel strains exclusively producing toxin A. A clinically significant strain, RT002, was detected in cats. Antimicrobial resistance was noted in numerous isolates from various sources. Several novel strains failed to produce black colonies on ChromID agar, exhibiting unique characteristics.

KW - Clostridioides (Clostridium) difficile

KW - Molecular Epidemiology

KW - Homes

KW - Novel Variant strains

KW - Phenotypic and Genotypic Characterisation

KW - Food and Environment

KW - OneHealth

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DO - 10.26182/5j7f-9e47

M3 - Doctoral Thesis

ER -

Clostridioides (Clostridium) difficile in the home environment in Perth, Western Australia

Abstract

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Research output

Complete Genome Sequences of Evolutionary Clade C-III Strains of Clostridioides (Clostridium) difficile Isolated from the Environment in Western Australia

The Brief Case: a White-Colony-Producing Clostridioides difficile Ribotype 020 Strain

Esculin hydrolysis negative and TcdA-only producing strains of Clostridium (Clostridiodes) difficile from the environment in Western Australia

Cite this