hShroom1 links a membrane bound protein to the actin cytoskeleton

D.E. Dye, S. Karlen, B. Rohrbach, O. Staub, L.R. Braathen, D.R. Coombe, Karin Eidne

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

hShroom1 (hShrm1) is a member of theApx/Shroom (Shrm) protein family and was identifiedfrom a yeast two-hybrid screen as a protein thatinteracts with the cytoplasmic domain of melanomacell adhesion molecule (MCAM). The characteristicsignature of the Shrm family is the presence of aunique domain, ASD2 (Apx/Shroom domain 2).mRNA analysis suggests that hShrm1 is expressed inbrain, heart, skeletal muscle, colon, small intestine,kidney, placenta and lung tissue, as well a variety ofmelanoma and other cell lines. Co-immunoprecipitationand bioluminescence resonance energy transfer(BRET) experiments indicate that hShrm1 andMCAM interact in vivo and by immunofluorescencemicroscopy some co-localization of these proteins isobserved. hShrm1 partly co-localises with b-actin andis found in the Triton X-100 insoluble fraction ofmelanoma cell extracts. We propose that hShrm1
Original languageEnglish
Pages (from-to)681-696
JournalCellular and Molecular Life Sciences
Volume66
Issue number4
DOIs
Publication statusPublished - 2009

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