@article{432ac6a850d1422e92ec76d7addf60b4,
title = "HPLC-UV assay of tramadol and O-desmethyltramadol in human plasma containing other drugs potentially co-administered to participants in a paediatric population pharmacokinetic study",
abstract = "Multimodal analgesia is employed in paediatric pain management to maximise analgesia and minimise side effects. Tramadol is dosed at 1–1.5 mg/kg to treat severe pain in children but the assay for tramadol in plasma samples for pharmacokinetic and toxicology studies does not often consider concurrently administered medications. In this study we developed and validated an HPLC–UV method to quantify tramadol and its main metabolite (O-desmethyltramadol) in human plasma in the presence of seven potentially interfering drugs. Sample preparation method was developed by combining liquid–liquid extraction and protein precipitation. Chromatographic separation was achieved on a BDS-Hypersil-C18 column (5 µm, 250 × 4.6 mm) using a double gradient method. The limit of quantification was 6.7 ng/ml for both tramadol and ODT. The precision and accuracy were in compliance with ICH guidelines. This method was successfully employed to analyse the blood samples of 137 paediatric participants in a tramadol pharmacokinetic trial.",
keywords = "HPLC assay, O-desmethyltramadol, Paediatrics pharmacokinetics, Plasma extraction, Population pharmacokinetics, Tramadol",
author = "O. Yoo and Tang, {E. K.Y.} and Nguyen, {M. N.} and S. Salman and Hua, {A. J.} and {von Ungern Sternberg}, {B. S.} and Lim, {L. Y.}",
note = "Funding Information: Funding from the Australian and New Zealand College of Anaesthetists (ANZCA) John Boyd Craig Research Award, Telethon-Perth Children's Hospital Research Fund, University of Western Australia Pathfinder Fund and Perth Children's Hospital Foundation (HPLC equipment) are gratefully acknowledged. Funding Information: Funding from the Australian and New Zealand College of Anaesthetists (ANZCA) John Boyd Craig Research Award, Telethon-Perth Children{\textquoteright}s Hospital Research Fund, University of Western Australia Pathfinder Fund and Perth Children{\textquoteright}s Hospital Foundation (HPLC equipment) are gratefully acknowledged. Publisher Copyright: {\textcopyright} 2021",
year = "2021",
month = nov,
day = "1",
doi = "10.1016/j.jchromb.2021.122971",
language = "English",
volume = "1184",
journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",
issn = "1570-0232",
publisher = "Elsevier",
}