House dust mites, associated bacteria and NlpC/P60-related bacteriolytic enzymes

Vivian Hui Ming Tang

doi:10.26182/5cd267dbb6882

House dust mites, associated bacteria and NlpC/P60-related bacteriolytic enzymes

Vivian Hui Ming Tang

School of Biomedical Sciences

Research output: Thesis › Doctoral Thesis

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Abstract

The bacteriolytic enzyme LytFM, isolated from the growth medium of house dust mites, shows high sequence identity to a group of bacterial NlpC/P60 proteins. This PhD investigated the hypothesis that LytFM might have originated from bacterial species cohabiting within the dust mite. Nine bacterial isolates from surface-sterilised dust mites were identified and three were found to contain the gene lytFM, while the variant protein LytFM1 was shown to be produced by these bacteria. Molecular and bioinformatic techniques indicated that lytFM!JytFM1 was recently acquired by the three dust-mite-derived bacterial species from their host, via cross-kingdom horizontal gene transfer.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	The University of Western Australia
Supervisors/Advisors	Peacock, Christopher, Supervisor Chang, Barbara, Supervisor Stewart, Geoffrey, Supervisor
Award date	14 Apr 2019
DOIs	https://doi.org/10.26182/5cd267dbb6882
Publication status	Unpublished - 2019

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10.26182/5cd267dbb6882

THESIS - DOCTOR OF PHILOSOPHY - TANG Vivian Hui Ming - 2019
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Cite this

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title = "House dust mites, associated bacteria and NlpC/P60-related bacteriolytic enzymes",

abstract = "The bacteriolytic enzyme LytFM, isolated from the growth medium of house dust mites, shows high sequence identity to a group of bacterial NlpC/P60 proteins. This PhD investigated the hypothesis that LytFM might have originated from bacterial species cohabiting within the dust mite. Nine bacterial isolates from surface-sterilised dust mites were identified and three were found to contain the gene lytFM, while the variant protein LytFM1 was shown to be produced by these bacteria. Molecular and bioinformatic techniques indicated that lytFM!JytFM1 was recently acquired by the three dust-mite-derived bacterial species from their host, via cross-kingdom horizontal gene transfer.",

keywords = "House dust mites, Dermatophagoides pteronyssinus, LytFM, Bacteriolytic enzymes, Bacillus licheniformis, horizontal gene transfer, NlpC/P60 superfamily, Microbes",

author = "Tang, {Vivian Hui Ming}",

year = "2019",

doi = "10.26182/5cd267dbb6882",

language = "English",

school = "The University of Western Australia",

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T1 - House dust mites, associated bacteria and NlpC/P60-related bacteriolytic enzymes

AU - Tang, Vivian Hui Ming

PY - 2019

Y1 - 2019

N2 - The bacteriolytic enzyme LytFM, isolated from the growth medium of house dust mites, shows high sequence identity to a group of bacterial NlpC/P60 proteins. This PhD investigated the hypothesis that LytFM might have originated from bacterial species cohabiting within the dust mite. Nine bacterial isolates from surface-sterilised dust mites were identified and three were found to contain the gene lytFM, while the variant protein LytFM1 was shown to be produced by these bacteria. Molecular and bioinformatic techniques indicated that lytFM!JytFM1 was recently acquired by the three dust-mite-derived bacterial species from their host, via cross-kingdom horizontal gene transfer.

AB - The bacteriolytic enzyme LytFM, isolated from the growth medium of house dust mites, shows high sequence identity to a group of bacterial NlpC/P60 proteins. This PhD investigated the hypothesis that LytFM might have originated from bacterial species cohabiting within the dust mite. Nine bacterial isolates from surface-sterilised dust mites were identified and three were found to contain the gene lytFM, while the variant protein LytFM1 was shown to be produced by these bacteria. Molecular and bioinformatic techniques indicated that lytFM!JytFM1 was recently acquired by the three dust-mite-derived bacterial species from their host, via cross-kingdom horizontal gene transfer.

KW - House dust mites

KW - Dermatophagoides pteronyssinus

KW - LytFM

KW - Bacteriolytic enzymes

KW - Bacillus licheniformis

KW - horizontal gene transfer

KW - NlpC/P60 superfamily

KW - Microbes

U2 - 10.26182/5cd267dbb6882

DO - 10.26182/5cd267dbb6882

M3 - Doctoral Thesis

ER -

House dust mites, associated bacteria and NlpC/P60-related bacteriolytic enzymes

Abstract

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