The bacteriolytic enzyme LytFM, isolated from the growth medium of house dust mites, shows high sequence identity to a group of bacterial NlpC/P60 proteins. This PhD investigated the hypothesis that LytFM might have originated from bacterial species cohabiting within the dust mite. Nine bacterial isolates from surface-sterilised dust mites were identified and three were found to contain the gene lytFM, while the variant protein LytFM1 was shown to be produced by these bacteria. Molecular and bioinformatic techniques indicated that lytFM!JytFM1 was recently acquired by the three dust-mite-derived bacterial species from their host, via cross-kingdom horizontal gene transfer.
|Qualification||Doctor of Philosophy|
|Award date||14 Apr 2019|
|Publication status||Unpublished - 2019|