Hls5 regulated erythroid differentiation by modulating GATA-1 activity

Raelene Endersby, I.J. Majewski, Louise Winteringham, Jennifer Beaumont, Amy Samuels, Robin Scaife, Esther Lim, M. Crossley, Peter Klinken, J.P. Lalonde

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Hemopoietic lineage switch (Hls) 5 and 7 were originally isolated as genes up-regulated during an erythroid-to-myeloid lineage switch. We have shown previously that Hls7/Mlf1 imposes a monoblastoid phenotype on erythroleukemic cells. Here we show that Hls5 impedes erythroid maturation by restricting proliferation and inhibiting hemoglobin synthesis; however, Hls5 does not influence the morphology of erythroid cells. Under the influence of GATA-1, Hls5 relocates from cytoplasmic granules to the nucleus where it associates with both FOG-1 and GATA-1. In the nucleus, Hls5 is able to suppress GATA-1–mediated transactivation and reduce GATA-1 binding to DNA. We conclude that Hls5 and Hls7/Mlf1 act cooperatively to induce biochemical and phenotypic changes associated with erythroid/myeloid lineage switching.
Original languageEnglish
Pages (from-to)1946-1950
JournalBlood
Volume111
Issue number4
DOIs
Publication statusPublished - 2008

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