The HLA-DRB1 sequencing based typing strategies reported to date require separate amplifications of each sample with a series of group-specific primers followed by sequencing of any resulting polymerase chain reaction (PCR) products. Whilst this results in high resolution typing in the majority of cases, a number of unnecessary amplifications are performed Me report here a novel approach where amplification of the second exon of HLA-DRB1 is performed in a single tube for all alleles. Retrospective analysis of 642 consecutive Western Australian unrelated bone marrow registry donors has shown that this approach results in unambiguous typings in 71.1% of cases Ambiguities can be readily resolved if necessary with a single additional sequencing reaction on the original PCR product.
|Publication status||Published - 2001|
Sayer, D., Whidborne, R., Brestovac, B., Trimboli, F., Witt, C., & Christiansen, F. (2001). HLA-DRB1 DNA sequencing based typing: an approach suitable for high throughput typing including unrelated bone marrow registry donors. Tissue Antigens, 57(N/A), 46-54. https://doi.org/10.1034/j.1399-0039.2001.057001046.x