High-Throughput Oxygen Consumption Measurements in Leaf Tissue Using Oxygen Sensitive Fluorophores

Research output: Chapter in Book/Conference paperChapterpeer-review

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Abstract

Respiratory rate measurements are crucial assays to understand mitochondrial biochemistry as well as metabolic regulation within tissues. Several technologies currently exist that can measure plant respiratory oxygen consumption or carbon dioxide evolution rates over short durations by either isolated mitochondria or plant tissues. Here we describe recently developed alternative methods for measuring tissue oxygen consumption rates (OCRs) using systems reliant on oxygen sensitive fluorophores. The methods described have distinct experimental advantages: they can allow high-throughput and long-duration measurements; and they are particularly suited to investigating the metabolic regulation of respiration by comparing OCRs among treatments or genotypes.

Original languageEnglish
Title of host publicationPlant Mitochondria
Subtitle of host publicationMethods and Protocols
EditorsOlivier Van Aken, Allan G. Rasmusson
Place of PublicationNY
PublisherHumana Press
Chapter6
Pages63-75
Number of pages13
ISBN (Electronic)978-1-0716-1653-6
ISBN (Print)978-1-0716-1652-9
DOIs
Publication statusPublished - 2022

Publication series

NameMethods in molecular biology (Clifton, N.J.)
PublisherHumana Press
Volume2363
ISSN (Print)1064-3745

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