High-Throughput BN-PAGE for Mitochondrial Respiratory Complexes

Lilian Vincis Pereira Sanglard, Catherine Colas des Francs-Small

Research output: Chapter in Book/Conference paperChapterpeer-review

4 Citations (Scopus)

Abstract

Blue native electrophoresis (BN-PAGE) is a highly resolutive method suited to the study of high molecular weight protein complexes between 100 and >3000 kDa. One of the drawbacks of this method is that it is very time-consuming and requires high quantities of purified organelles. Here we describe a high throughput BN-PAGE method allowing to screen libraries of plants potentially altered in respiratory metabolism.

Original languageEnglish
Title of host publicationPlant Mitochondria
Subtitle of host publicationMethods and Protocols
EditorsOlivier Van Aken, Allan G. Rasmusson
PublisherHumana Press Inc.
Chapter10
Pages111-119
Number of pages9
ISBN (Electronic)978-1-0716-1653-6
ISBN (Print)978-1-0716-1652-9
DOIs
Publication statusPublished - 2022

Publication series

NameMethods in Molecular Biology
Volume2363
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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