High-resolution, wide-field object reconstruction with synthetic aperture Fourier holographic optical microscopy

Timothy Hillman, Thomas Gutzler, Sergey Alexandrov, David Sampson

    Research output: Contribution to journalArticle

    112 Citations (Scopus)

    Abstract

    We utilize synthetic-aperture Fourier holographic microscopy to resolve micrometer-scale microstructure over millimeter-scale fields of view. Multiple holograms are recorded, each registering a different, limited region of the sample object’s Fourier spectrum. They are “stitched together” to generate the synthetic aperture. A low-numerical-aperture (NA) objective lens provides the wide field of view, and the additional advantages of a long working distance, no immersion fluids, and an inexpensive, simple optical system. Following the first theoretical treatment of the technique, we present images of a microchip target derived from an annular synthetic aperture (NA = 0.61) whose area is 15 times that due to a single hologram (NA = 0.13); they exhibit a corresponding qualitative improvement. We demonstrate that a high-quality reconstruction may be obtained from a limited sub-region of Fourier space, if the object’s structural information is concentrated there.© 2009 Optical Society of America
    Original languageEnglish
    Pages (from-to)7873-7892
    JournalOptics Express
    Volume17
    Issue number10
    DOIs
    Publication statusPublished - 2009

    Fingerprint Dive into the research topics of 'High-resolution, wide-field object reconstruction with synthetic aperture Fourier holographic optical microscopy'. Together they form a unique fingerprint.

    Cite this