High-resolution sub-cellular imaging by correlative NanoSIMS and electron microscopy of amiodarone internalisation by lung macrophages as evidence for drug-induced phospholipidosis

Haibo Jiang; Melissa K. Passarelli; Peter M G Munro; Matt R. Kilburn; Andrew West; Colin T. Dollery; Ian S. Gilmore; Paulina D. Rakowska

doi:10.1039/c6cc08549k

High-resolution sub-cellular imaging by correlative NanoSIMS and electron microscopy of amiodarone internalisation by lung macrophages as evidence for drug-induced phospholipidosis

Haibo Jiang, Melissa K. Passarelli, Peter M G Munro, Matt R. Kilburn, Andrew West, Colin T. Dollery, Ian S. Gilmore, Paulina D. Rakowska

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

Correlative NanoSIMS and EM imaging of amiodarone-treated macrophages shows the internalisation of the drug at a sub-cellular level and reveals its accumulation within the lysosomes, providing direct evidence for amiodarone-induced phospholipidosis. Chemical fixation using tannic acid effectively seals cellular membranes aiding intracellular retention of diffusible drugs.

Original language	English
Pages (from-to)	1506-1509
Number of pages	4
Journal	Chemical Communications
Volume	53
Issue number	9
DOIs	https://doi.org/10.1039/c6cc08549k https://doi.org/DOI: 10.1039/c6cc08549k
Publication status	Published - 2017

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Jiang, H., Passarelli, M. K., Munro, P. M. G., Kilburn, M. R., West, A., Dollery, C. T., Gilmore, I. S., & Rakowska, P. D. (2017). High-resolution sub-cellular imaging by correlative NanoSIMS and electron microscopy of amiodarone internalisation by lung macrophages as evidence for drug-induced phospholipidosis. Chemical Communications, 53(9), 1506-1509. https://doi.org/10.1039/c6cc08549k, https://doi.org/DOI: 10.1039/c6cc08549k

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title = "High-resolution sub-cellular imaging by correlative NanoSIMS and electron microscopy of amiodarone internalisation by lung macrophages as evidence for drug-induced phospholipidosis",

abstract = "Correlative NanoSIMS and EM imaging of amiodarone-treated macrophages shows the internalisation of the drug at a sub-cellular level and reveals its accumulation within the lysosomes, providing direct evidence for amiodarone-induced phospholipidosis. Chemical fixation using tannic acid effectively seals cellular membranes aiding intracellular retention of diffusible drugs.",

author = "Haibo Jiang and Passarelli, {Melissa K.} and Munro, {Peter M G} and Kilburn, {Matt R.} and Andrew West and Dollery, {Colin T.} and Gilmore, {Ian S.} and Rakowska, {Paulina D.}",

year = "2017",

doi = "10.1039/c6cc08549k",

language = "English",

volume = "53",

pages = "1506--1509",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "ROYAL SOC CHEMISTRY",

number = "9",

}

Jiang, H, Passarelli, MK, Munro, PMG, Kilburn, MR, West, A, Dollery, CT, Gilmore, IS & Rakowska, PD 2017, 'High-resolution sub-cellular imaging by correlative NanoSIMS and electron microscopy of amiodarone internalisation by lung macrophages as evidence for drug-induced phospholipidosis', Chemical Communications, vol. 53, no. 9, pp. 1506-1509. https://doi.org/10.1039/c6cc08549k, https://doi.org/DOI: 10.1039/c6cc08549k

High-resolution sub-cellular imaging by correlative NanoSIMS and electron microscopy of amiodarone internalisation by lung macrophages as evidence for drug-induced phospholipidosis. / Jiang, Haibo; Passarelli, Melissa K.; Munro, Peter M G et al.
In: Chemical Communications, Vol. 53, No. 9, 2017, p. 1506-1509.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

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AU - Jiang, Haibo

AU - Passarelli, Melissa K.

AU - Munro, Peter M G

AU - Kilburn, Matt R.

AU - West, Andrew

AU - Dollery, Colin T.

AU - Gilmore, Ian S.

AU - Rakowska, Paulina D.

PY - 2017

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AB - Correlative NanoSIMS and EM imaging of amiodarone-treated macrophages shows the internalisation of the drug at a sub-cellular level and reveals its accumulation within the lysosomes, providing direct evidence for amiodarone-induced phospholipidosis. Chemical fixation using tannic acid effectively seals cellular membranes aiding intracellular retention of diffusible drugs.

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DO - 10.1039/c6cc08549k

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SN - 1359-7345

VL - 53

SP - 1506

EP - 1509

JO - Chemical Communications

JF - Chemical Communications

IS - 9

ER -

High-resolution sub-cellular imaging by correlative NanoSIMS and electron microscopy of amiodarone internalisation by lung macrophages as evidence for drug-induced phospholipidosis

Abstract

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High-resolution sub-cellular imaging by correlative NanoSIMS and electron microscopy of amiodarone internalisation by lung macrophages as evidence for drug-induced phospholipidosis

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