High-resolution sub-cellular imaging by correlative NanoSIMS and electron microscopy of amiodarone internalisation by lung macrophages as evidence for drug-induced phospholipidosis

Haibo Jiang, Melissa K. Passarelli, Peter M G Munro, Matt R. Kilburn, Andrew West, Colin T. Dollery, Ian S. Gilmore, Paulina D. Rakowska

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Correlative NanoSIMS and EM imaging of amiodarone-treated macrophages shows the internalisation of the drug at a sub-cellular level and reveals its accumulation within the lysosomes, providing direct evidence for amiodarone-induced phospholipidosis. Chemical fixation using tannic acid effectively seals cellular membranes aiding intracellular retention of diffusible drugs.

Original languageEnglish
Pages (from-to)1506-1509
Number of pages4
JournalChemical Communications
Volume53
Issue number9
DOIs
Publication statusPublished - 2017

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