TY - JOUR
T1 - High-resolution skim genotyping by sequencing reveals the distribution of crossovers and gene conversions in Cicer arietinum and Brassica napus
AU - Bayer, Philipp
AU - Ruperao, P.
AU - Mason, A.S.
AU - Stiller, J.
AU - Chan, Kenneth
AU - Hayashi, S.
AU - Long, Y.
AU - Meng, J.
AU - Sutton, T.
AU - Visendi, P.
AU - Varshney, Rajeev K.
AU - Batley, Jacqueline
AU - Edwards, Dave
PY - 2015/6
Y1 - 2015/6
N2 - © 2015, Springer-Verlag Berlin Heidelberg. Key message: We characterise the distribution of crossover and non-crossover recombination inBrassica napusandCicer arietinumusing a low-coverage genotyping by sequencing pipeline SkimGBS. Abstract: The growth of next-generation DNA sequencing technologies has led to a rapid increase in sequence-based genotyping for applications including diversity assessment, genome structure validation and gene–trait association. We have established a skim-based genotyping by sequencing method for crop plants and applied this approach to genotype-segregating populations of Brassica napus and Cicer arietinum. Comparison of progeny genotypes with those of the parental individuals allowed the identification of crossover and non-crossover (gene conversion) events. Our results identify the positions of recombination events with high resolution, permitting the mapping and frequency assessment of recombination in segregating populations.
AB - © 2015, Springer-Verlag Berlin Heidelberg. Key message: We characterise the distribution of crossover and non-crossover recombination inBrassica napusandCicer arietinumusing a low-coverage genotyping by sequencing pipeline SkimGBS. Abstract: The growth of next-generation DNA sequencing technologies has led to a rapid increase in sequence-based genotyping for applications including diversity assessment, genome structure validation and gene–trait association. We have established a skim-based genotyping by sequencing method for crop plants and applied this approach to genotype-segregating populations of Brassica napus and Cicer arietinum. Comparison of progeny genotypes with those of the parental individuals allowed the identification of crossover and non-crossover (gene conversion) events. Our results identify the positions of recombination events with high resolution, permitting the mapping and frequency assessment of recombination in segregating populations.
UR - https://www.scopus.com/pages/publications/84938890107
U2 - 10.1007/s00122-015-2488-y
DO - 10.1007/s00122-015-2488-y
M3 - Article
C2 - 25754422
SN - 0040-5752
VL - 128
SP - 1039
EP - 1047
JO - Theoretical and Applied Genetics
JF - Theoretical and Applied Genetics
IS - 6
ER -