High-resolution skim genotyping by sequencing reveals the distribution of crossovers and gene conversions in Cicer arietinum and Brassica napus

Philipp Bayer, P. Ruperao, A.S. Mason, J. Stiller, Kenneth Chan, S. Hayashi, Y. Long, J. Meng, T. Sutton, P. Visendi, Rajeev K. Varshney, Jacqueline Batley, Dave Edwards

    Research output: Contribution to journalArticle

    37 Citations (Scopus)

    Abstract

    © 2015, Springer-Verlag Berlin Heidelberg. Key message: We characterise the distribution of crossover and non-crossover recombination inBrassica napusandCicer arietinumusing a low-coverage genotyping by sequencing pipeline SkimGBS. Abstract: The growth of next-generation DNA sequencing technologies has led to a rapid increase in sequence-based genotyping for applications including diversity assessment, genome structure validation and gene–trait association. We have established a skim-based genotyping by sequencing method for crop plants and applied this approach to genotype-segregating populations of Brassica napus and Cicer arietinum. Comparison of progeny genotypes with those of the parental individuals allowed the identification of crossover and non-crossover (gene conversion) events. Our results identify the positions of recombination events with high resolution, permitting the mapping and frequency assessment of recombination in segregating populations.
    Original languageEnglish
    Pages (from-to)1039-1047
    JournalTheoretical and Applied Genetics
    Volume128
    Issue number6
    Early online date10 Mar 2015
    DOIs
    Publication statusPublished - Jun 2015

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