HIGH-LEVEL EXPRESSION OF A HETEROLOGOUS PROTEIN IN LACTOBACILLUS-PLANTARUM AND ITS EFFECT ON THE PERSISTENCE OF THE RECOMBINANT STRAIN IN SILAGE

JE Rixon; R Sharp; A.G. O'Donnell; GP Hazlewood; JK Brehm; NP Minton; HJ Gilbert

doi:10.1007/BF00129377

HIGH-LEVEL EXPRESSION OF A HETEROLOGOUS PROTEIN IN LACTOBACILLUS-PLANTARUM AND ITS EFFECT ON THE PERSISTENCE OF THE RECOMBINANT STRAIN IN SILAGE

JE Rixon
, R Sharp
, A.G. O'Donnell
, GP Hazlewood
, JK Brehm
, NP Minton
, HJ Gilbert

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

This paper reports the high level expression of the Staphylococcus aureus cat gene in Lactobacillus plantarum using the expression vector pMTL500F. When the recombinant strain of L. plantarum was grown in pure culture, CAT contributed 1.4% of the total soluble cell protein. The recombinant strain of L. plantarum continued to express a high level of CAT when inoculated into silage, the heterologous protein constituting up to 1.75% of the total soluble cell protein. The recombinant L. plantarum strain was still able to survive and proliferate when inoculated into silage, despite its additional metabolic load.

Original language	English
Pages (from-to)	561-566
Journal	Biotechnology Letters
Volume	17
DOIs	https://doi.org/10.1007/BF00129377
Publication status	Published - 1995

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title = "HIGH-LEVEL EXPRESSION OF A HETEROLOGOUS PROTEIN IN LACTOBACILLUS-PLANTARUM AND ITS EFFECT ON THE PERSISTENCE OF THE RECOMBINANT STRAIN IN SILAGE",

abstract = "This paper reports the high level expression of the Staphylococcus aureus cat gene in Lactobacillus plantarum using the expression vector pMTL500F. When the recombinant strain of L. plantarum was grown in pure culture, CAT contributed 1.4\% of the total soluble cell protein. The recombinant strain of L. plantarum continued to express a high level of CAT when inoculated into silage, the heterologous protein constituting up to 1.75\% of the total soluble cell protein. The recombinant L. plantarum strain was still able to survive and proliferate when inoculated into silage, despite its additional metabolic load.",

author = "JE Rixon and R Sharp and A.G. O'Donnell and GP Hazlewood and JK Brehm and NP Minton and HJ Gilbert",

year = "1995",

doi = "10.1007/BF00129377",

language = "English",

volume = "17",

pages = "561--566",

journal = "Biotechnology Letters",

issn = "0141-5492",

publisher = "Springer Science and Business Media B.V.",

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TY - JOUR

T1 - HIGH-LEVEL EXPRESSION OF A HETEROLOGOUS PROTEIN IN LACTOBACILLUS-PLANTARUM AND ITS EFFECT ON THE PERSISTENCE OF THE RECOMBINANT STRAIN IN SILAGE

AU - Rixon, JE

AU - Sharp, R

AU - O'Donnell, A.G.

AU - Hazlewood, GP

AU - Brehm, JK

AU - Minton, NP

AU - Gilbert, HJ

PY - 1995

Y1 - 1995

N2 - This paper reports the high level expression of the Staphylococcus aureus cat gene in Lactobacillus plantarum using the expression vector pMTL500F. When the recombinant strain of L. plantarum was grown in pure culture, CAT contributed 1.4% of the total soluble cell protein. The recombinant strain of L. plantarum continued to express a high level of CAT when inoculated into silage, the heterologous protein constituting up to 1.75% of the total soluble cell protein. The recombinant L. plantarum strain was still able to survive and proliferate when inoculated into silage, despite its additional metabolic load.

AB - This paper reports the high level expression of the Staphylococcus aureus cat gene in Lactobacillus plantarum using the expression vector pMTL500F. When the recombinant strain of L. plantarum was grown in pure culture, CAT contributed 1.4% of the total soluble cell protein. The recombinant strain of L. plantarum continued to express a high level of CAT when inoculated into silage, the heterologous protein constituting up to 1.75% of the total soluble cell protein. The recombinant L. plantarum strain was still able to survive and proliferate when inoculated into silage, despite its additional metabolic load.

U2 - 10.1007/BF00129377

DO - 10.1007/BF00129377

M3 - Article

SN - 0141-5492

VL - 17

SP - 561

EP - 566

JO - Biotechnology Letters

JF - Biotechnology Letters

ER -

HIGH-LEVEL EXPRESSION OF A HETEROLOGOUS PROTEIN IN LACTOBACILLUS-PLANTARUM AND ITS EFFECT ON THE PERSISTENCE OF THE RECOMBINANT STRAIN IN SILAGE

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