Hepcidin expression inversely correlates with the expression of duodenal iron transporters and iron absorption in rats

D.M. Frazer, S.J. Wilkins, E.M. Becker, C.D. Vulpe, A.T. Mckie, Debbie Trinder, G.J. Anderson

    Research output: Contribution to journalArticle

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    Abstract

    Background & Aims: Hepcidin is an antimicrobial peptide thought to be involved in the regulation of intestinal iron absorption. To further investigate its role in this process, we examined hepatic and duodenal gene expression in rats after the switch from a control diet to an iron-deficient diet. Methods: Adult rats on an iron-replete diet were switched to an iron-deficient diet and the expression of iron homeostasis molecules in duodenal and liver tissue was studied over 14 days. Intestinal iron absorption was determined at these same time-points by measuring the retention of an oral dose of Fe-59. Results: Iron absorption increased 2.7-fold within 6 days of switching to an iron-deficient diet and was accompanied by an increase in the duodenal expression of Dcytb, divalent metal transporter 1, and Ireg1. These changes precisely correlated with decreases in hepatic hepcidin expression and transferrin saturation. No change in iron stores or hematologic parameters was detected. Conclusions: This study showed a close relationship between the expression of hepcidin, duodenal iron transporters, and iron absorption. Both hepcidin expression and iron absorption can be regulated before iron stores and erythropoiesis are affected, and transferrin saturation may signal such changes.
    Original languageEnglish
    Pages (from-to)835-844
    JournalGastroenterology
    Volume123
    Issue number3
    DOIs
    Publication statusPublished - 2002

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    Hepcidins
    Iron
    Diet
    Intestinal Absorption
    Transferrin
    Liver
    Erythropoiesis

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    Frazer, D.M. ; Wilkins, S.J. ; Becker, E.M. ; Vulpe, C.D. ; Mckie, A.T. ; Trinder, Debbie ; Anderson, G.J. / Hepcidin expression inversely correlates with the expression of duodenal iron transporters and iron absorption in rats. In: Gastroenterology. 2002 ; Vol. 123, No. 3. pp. 835-844.
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    abstract = "Background & Aims: Hepcidin is an antimicrobial peptide thought to be involved in the regulation of intestinal iron absorption. To further investigate its role in this process, we examined hepatic and duodenal gene expression in rats after the switch from a control diet to an iron-deficient diet. Methods: Adult rats on an iron-replete diet were switched to an iron-deficient diet and the expression of iron homeostasis molecules in duodenal and liver tissue was studied over 14 days. Intestinal iron absorption was determined at these same time-points by measuring the retention of an oral dose of Fe-59. Results: Iron absorption increased 2.7-fold within 6 days of switching to an iron-deficient diet and was accompanied by an increase in the duodenal expression of Dcytb, divalent metal transporter 1, and Ireg1. These changes precisely correlated with decreases in hepatic hepcidin expression and transferrin saturation. No change in iron stores or hematologic parameters was detected. Conclusions: This study showed a close relationship between the expression of hepcidin, duodenal iron transporters, and iron absorption. Both hepcidin expression and iron absorption can be regulated before iron stores and erythropoiesis are affected, and transferrin saturation may signal such changes.",
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    Hepcidin expression inversely correlates with the expression of duodenal iron transporters and iron absorption in rats. / Frazer, D.M.; Wilkins, S.J.; Becker, E.M.; Vulpe, C.D.; Mckie, A.T.; Trinder, Debbie; Anderson, G.J.

    In: Gastroenterology, Vol. 123, No. 3, 2002, p. 835-844.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Hepcidin expression inversely correlates with the expression of duodenal iron transporters and iron absorption in rats

    AU - Frazer, D.M.

    AU - Wilkins, S.J.

    AU - Becker, E.M.

    AU - Vulpe, C.D.

    AU - Mckie, A.T.

    AU - Trinder, Debbie

    AU - Anderson, G.J.

    PY - 2002

    Y1 - 2002

    N2 - Background & Aims: Hepcidin is an antimicrobial peptide thought to be involved in the regulation of intestinal iron absorption. To further investigate its role in this process, we examined hepatic and duodenal gene expression in rats after the switch from a control diet to an iron-deficient diet. Methods: Adult rats on an iron-replete diet were switched to an iron-deficient diet and the expression of iron homeostasis molecules in duodenal and liver tissue was studied over 14 days. Intestinal iron absorption was determined at these same time-points by measuring the retention of an oral dose of Fe-59. Results: Iron absorption increased 2.7-fold within 6 days of switching to an iron-deficient diet and was accompanied by an increase in the duodenal expression of Dcytb, divalent metal transporter 1, and Ireg1. These changes precisely correlated with decreases in hepatic hepcidin expression and transferrin saturation. No change in iron stores or hematologic parameters was detected. Conclusions: This study showed a close relationship between the expression of hepcidin, duodenal iron transporters, and iron absorption. Both hepcidin expression and iron absorption can be regulated before iron stores and erythropoiesis are affected, and transferrin saturation may signal such changes.

    AB - Background & Aims: Hepcidin is an antimicrobial peptide thought to be involved in the regulation of intestinal iron absorption. To further investigate its role in this process, we examined hepatic and duodenal gene expression in rats after the switch from a control diet to an iron-deficient diet. Methods: Adult rats on an iron-replete diet were switched to an iron-deficient diet and the expression of iron homeostasis molecules in duodenal and liver tissue was studied over 14 days. Intestinal iron absorption was determined at these same time-points by measuring the retention of an oral dose of Fe-59. Results: Iron absorption increased 2.7-fold within 6 days of switching to an iron-deficient diet and was accompanied by an increase in the duodenal expression of Dcytb, divalent metal transporter 1, and Ireg1. These changes precisely correlated with decreases in hepatic hepcidin expression and transferrin saturation. No change in iron stores or hematologic parameters was detected. Conclusions: This study showed a close relationship between the expression of hepcidin, duodenal iron transporters, and iron absorption. Both hepcidin expression and iron absorption can be regulated before iron stores and erythropoiesis are affected, and transferrin saturation may signal such changes.

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