Harnessing single nucleotide polymorphisms for Brassica napus breeding: an empirical evaluation of genotyping by sequencing and development of a web database for genotyping array markers

Armin Patrick Scheben

Research output: ThesisDoctoral Thesis

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Abstract

Genotyping by sequencing (GBS) and genotyping arrays have produced a deluge of data on single nucleotide polymorphisms (SNPs) in crops. By applying three GBS approaches to three Brassica napus F2 mapping populations and a diversity panel, we show that GBS efficiency is on par with genotyping arrays. We use GBS markers to develop linkage maps and identify a quantitative trait locus for flowering time. Further, we present CropSNPdb, a database for SNPs generated using genotyping arrays. Our growing genotyping capacity, together with public crop databases, will help breeders harness genomic variation to ensure food security.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • The University of Western Australia
Supervisors/Advisors
  • Finnegan, Patrick, Supervisor
  • Bayer, Philipp, Supervisor
  • Batley, Jacqueline, Supervisor
  • Edwards, Dave, Supervisor
Thesis sponsors
Award date13 Sept 2019
DOIs
Publication statusUnpublished - 2019

Embargo information

  • Embargoed from 17/09/2019 to 17/09/2021. Made publicly available on 17/09/2021.

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