TY - CHAP
T1 - Harnessing CRISPR/Cas System for Resistance against Abiotic Stresses in Rice
T2 - Recent Advances, Applications, Challenges, and Prospects
AU - Ahmad, Shakeel
AU - Nisar, Amina
AU - Jamil, Shakra
AU - Shahzad, Rahil
AU - Sheng, Zhonghua
AU - Iqbal, Muhammad Munir
AU - Wei, Chen
AU - Hu, Shikai
AU - Ju, Luo
AU - Wei, Xiangjin
AU - Hu, Peisong
AU - Tang, Shaoqing
N1 - Publisher Copyright:
© 2022 by Apple Academic Press, Inc.
PY - 2022/1/1
Y1 - 2022/1/1
N2 - Half of the world’s population consume rice (Oryza sativa L.) as staple food. The last couple of decades have witnessed a tremendous increase in the productivity of the rice by using the conventional, mutation, and molecular breeding approaches for development of elite rice cultivars. Nevertheless, on the same time, several biotic and abiotic stresses are putting continuous pressure on rice cultivars hampering their yield potential and lowering productivity, and ultimately threating the global food security. In this changing climate scenario, there is an utmost need to breed for climate resilient rice cultivars to feed the ever-growing population worldwide that is expected to reach 9.7 billion by 2050. Latest developments in the field of functional genomics have revolutionized agriculture and plant sciences. The greatest landmark of the last decade in this respect is the advent of CRISPR/Cas9 genome editing system. It is widely accepted and most advanced form of genome editing due to high efficiency, robustness, and simplicity. Owing to model organism for monocot and rich genomic resources coupled with small genome size makes rice more suitable crop for genetic manipulation. In this book chapter, we have focused on the genome editing strategies for rice improvement, thereby highlighting the applications and advancements of CRISPR/Cas9 system. We also shed light on the role of CRISPR/Cpf1, base editors, and prime editors in the field of genome editing highlighting major challenges and future implications of these tools in rice improvement.
AB - Half of the world’s population consume rice (Oryza sativa L.) as staple food. The last couple of decades have witnessed a tremendous increase in the productivity of the rice by using the conventional, mutation, and molecular breeding approaches for development of elite rice cultivars. Nevertheless, on the same time, several biotic and abiotic stresses are putting continuous pressure on rice cultivars hampering their yield potential and lowering productivity, and ultimately threating the global food security. In this changing climate scenario, there is an utmost need to breed for climate resilient rice cultivars to feed the ever-growing population worldwide that is expected to reach 9.7 billion by 2050. Latest developments in the field of functional genomics have revolutionized agriculture and plant sciences. The greatest landmark of the last decade in this respect is the advent of CRISPR/Cas9 genome editing system. It is widely accepted and most advanced form of genome editing due to high efficiency, robustness, and simplicity. Owing to model organism for monocot and rich genomic resources coupled with small genome size makes rice more suitable crop for genetic manipulation. In this book chapter, we have focused on the genome editing strategies for rice improvement, thereby highlighting the applications and advancements of CRISPR/Cas9 system. We also shed light on the role of CRISPR/Cpf1, base editors, and prime editors in the field of genome editing highlighting major challenges and future implications of these tools in rice improvement.
KW - Abiotic stress
KW - Climate change
KW - CRISPR/Cas system
KW - Genome editing
KW - Rice
UR - http://www.scopus.com/inward/record.url?scp=85131998687&partnerID=8YFLogxK
U2 - 10.1201/9781003180579-10
DO - 10.1201/9781003180579-10
M3 - Chapter
AN - SCOPUS:85131998687
SN - 9781774630181
SP - 241
EP - 261
BT - Plant Abiotic Stress Physiology
PB - Apple Academic Press
ER -