Abstract
| Original language | English |
|---|---|
| Pages (from-to) | online - approx 5-20pp |
| Journal | Histochemistry and Cell Biology |
| Volume | 125 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2006 |
Fingerprint
Cite this
}
Haemochromatosis protein is expressed on the terminal web of enterocytes in proximal small intestine of the rat. / West, Adrian; Thomas, Carla; Sadleir, John; Oates, Phillip.
In: Histochemistry and Cell Biology, Vol. 125, No. 3, 2006, p. online - approx 5-20pp.Research output: Contribution to journal › Article
TY - JOUR
T1 - Haemochromatosis protein is expressed on the terminal web of enterocytes in proximal small intestine of the rat
AU - West, Adrian
AU - Thomas, Carla
AU - Sadleir, John
AU - Oates, Phillip
PY - 2006
Y1 - 2006
N2 - The haemochromatosis protein (HFE) is an important regulator of body iron stores. In the liver, HFE is required for appropriate expression of hepcidin, a humoral mediator of iron absorption. HFE is also present in enterocytes, though its function in the intestine is unknown; it is not intrinsically required for iron absorption, but can augment iron absorption when over-expressed-independent of hepcidin regulation by the liver. In this study, an antibody was raised against rat HFE and validated by enzyme-linked immunosorbent assay, Western blot and quenching of antibody function by the immunising peptide. The sub-cellular location of HFE in enterocytes of iron-deficient and control rats was determined by double-labelling experiments with markers for the microvillus membrane, terminal web, early endosomes, lysosomes and the transferrin receptor. Parallel studies were performed for the primary iron absorption protein, divalent metal transporter 1 (DMT1). HFE co-localised exclusively with the terminal web of intestinal enterocytes. HFE expression was increased in iron deficiency, consistent with a second regulatory role for HFE in iron absorption, independent of hepcidin from the liver. DMT1 was localised primarily on the microvillus membrane, but did partially co-localise with HFE raising the possibility that the two proteins may interact to regulate iron absorption.
AB - The haemochromatosis protein (HFE) is an important regulator of body iron stores. In the liver, HFE is required for appropriate expression of hepcidin, a humoral mediator of iron absorption. HFE is also present in enterocytes, though its function in the intestine is unknown; it is not intrinsically required for iron absorption, but can augment iron absorption when over-expressed-independent of hepcidin regulation by the liver. In this study, an antibody was raised against rat HFE and validated by enzyme-linked immunosorbent assay, Western blot and quenching of antibody function by the immunising peptide. The sub-cellular location of HFE in enterocytes of iron-deficient and control rats was determined by double-labelling experiments with markers for the microvillus membrane, terminal web, early endosomes, lysosomes and the transferrin receptor. Parallel studies were performed for the primary iron absorption protein, divalent metal transporter 1 (DMT1). HFE co-localised exclusively with the terminal web of intestinal enterocytes. HFE expression was increased in iron deficiency, consistent with a second regulatory role for HFE in iron absorption, independent of hepcidin from the liver. DMT1 was localised primarily on the microvillus membrane, but did partially co-localise with HFE raising the possibility that the two proteins may interact to regulate iron absorption.
U2 - 10.1007/s00418-005-0060-6
DO - 10.1007/s00418-005-0060-6
M3 - Article
VL - 125
SP - online - approx 5-20pp
JO - Histochemistry and Cell Biology
JF - Histochemistry and Cell Biology
SN - 0948-6143
IS - 3
ER -